葛根醇提物对肥胖型型糖尿病大血管损伤大鼠血清sVCAM.docVIP

葛根醇提物对肥胖?型2型糖尿病大血?管损伤大鼠血清s?VCAM葛根醇?提物对肥胖型2型?糖尿病大血管损伤?大鼠血清sVCA?M作者：邓路娟? 闫铭 庞宗然 ?李晓军 【摘要?】 目?的 通过观察葛根?醇提物对肥胖型2?型糖尿病（t2d?m）大血管损伤大?鼠血清可溶性血管?细胞黏附分子（s?vcam-1）、?血浆肿瘤坏死因子?（tnf-α）含?量变化的影响，探?讨其对t2dm大?血管损伤保护作用?的部分机制。方法? 雄性wista?r大鼠30只，每?日用自制的脂肪乳?1 ml/100? g灌胃，3周后?挑选总胆固醇（t?c）、甘油三酯(?tg)、低密度脂?蛋白（ldl）增?高，胰岛素敏感指?数下降的肥胖大鼠?，空腹6 h，尾?静脉快速注射链脲?佐菌素(stz)?30 mg/kg?，1周后血糖值高?于1 6.7? mmol/l的?大鼠作为t2dm?模型，于成模后3?周及6周观察胸主?动脉弓的病理改变?，证实t2dm大?血管损伤模型复制?成功。成模大鼠随?机分为葛根醇提物?组、盐酸二甲双胍?组和模型组3组，?每组各10只。另?取8只正常大鼠为?对照组。各组分别?给予葛根醇提物0?.9 g/kg；?盐酸二甲双胍0.?09 g/kg及?等剂量自来水，治?疗4周后处死各组?动物，取血清、血?浆分别测定svc?am- 1、?tnf-α的含量?。结果 模型组大?鼠血清svcam?- 1、血浆?tnf-α较正常?对照组上调，葛根?醇提物组及盐酸二?甲双胍组大鼠血清?svcam- ? 1、血浆tnf?-α的含量均显著?降低（p 0.0?5）。WWw..?Com结论 葛根?醇提物能降低t2?dm血管并发症大?鼠过高的血清sv?cam- 1?、血浆tnf-α?的水平，有助于控?制t2dm血管并?发症的发生发展。? ? 【关键词】? ? 葛根 ?浸膏制剂(中药)? 糖尿病 2型 ?胞间粘附分子 肿?瘤坏死因子α 血?管 损伤 【a?bstract】? objecti?ve to ob?serve th?e effect? of alco?holic ex?tract fr?om radix? puerari?ae on so?luble va?scular c?ell adhe?sion mol?ecule 1 ?(svcam-1?)and tnf?-αin ser?um of ra?ts with ?large ve?ssel inj?ury of o?bese t2m?d, and a?pproach ?the mech?anism of? prevent?ing larg?e vessel? injury ?of obese? t2md. m?ethod 30? wistar ?rats wer?e fed by? fat mil?k of 1ml?/100g. a?fter thr?ee weeks? adiposi?tas wist?ar rats ?with hig?h levels? of tc, ?tg and l?dl, and ?low leve?l of ins?ulin sen?sitivity? index (?isi) wer?e select?ed from ?30 wista?r rats. ?t2dm mod?el of th?ese rats? were es?tablishe?d by ven?a caudal?is injec?tion of ?stz, 30 ?mg/kg. r?ats mode?l with l?arge ves?sel inju?ry of ob?ese t2md? were co?nfirmed ?by obser?vation o?f pathol?ogical c?hange of? aorta t?horacica? arcual.? these m?odel rat?s were r?andomly ?divided ?into alc?oholic e?xtract f?rom radi?x puerar?iae grou?p (recei?ve alcoh?olic ext?ract fro?m radix ?pueraria?e, 0.9 g?/kg), me?tformin ?group (r?eceive m?etformin?, 0.09g/?kg) and ?model gr?oup (rec?eive tap?-water).? 8 norma?l rats w?ere as c?ontrol g?roup. af?ter four? weeks, ?these ra?ts were ?executed? and con?t