白吻动胸龟和剃刀麝香龟线粒体全序列比较及动胸龟科在龟鳖目中的系统发生位置（已处理）.doc

The complete mitochondrial genomes of Kinosternon leucostomum and Sternotherus carinatus and the phylogenetic position of Kinosternidae within Testudines 5 10 15 20 25 30 35 40 Jing Wanxing12 Zhang Yanyun12 Nie Liuwang12 Dai Xueting12 Xia Xingquan12 Huang Zhengfeng12 Wang Ling12 Jiang Yuan12 Liu Luo12 1 Life Science College Anhui Normal University Anhui Wuhu 241000 2 the Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui Wuhu 241000 China Abstract We first determined two complete mitochondrial genomes of Kinosternidae Kinosternon leucostomum 16 559bp and Sternotherus carinatus 16 554bp by primer walking and long-PCR technique which were deposited in the GenBank database under the accession number FJ915117 and HQ114563 respectively In the two sequences we identified three distinctive features 1 a 31bp nucleotides intergenic regions located between the COX1 and tRNASer UCN genes but found so far generally overlapped by 9bp or 5bp in other turtle families 2 a 10bp spacer between tRNACys and tRNATyr while most other turtle families had no spacers 3 a single nucleotide insertion in the ND4 gene of S carinatus which was rarely observed in other turtles The phylogenetic analyses based on the 12 mtDNA protein genes indicated that the ML and BI trees all showed K leucostomum and S carinatus assembled together to form a clade of the Kinosternidae which was sister group to the Chelydridae and the assemblage of Kinosternidae Chelydridae had a sister-taxon relationship to the Cheloniidae Testudinoidea clade Although the Asian box turtle and the kinosternids all had the kinetic plastron with the hinge it was found that the Asian box turtle was not close to the Kinosternidae indicating that the hinge structure might not be derived from one common ancestor Key words Kinosternidae mitochondrial genomes phylogenetic the kinetic plastron 1 Instruction The diversity of turtles in the world that have