内关穴位注射香丹注射液预处理对大鼠急性心肌缺血crp tnf-ɑ vegf的影响-effect of pretreatment of neiguan acupoint injection with xiangdan injection on crp tnf - α vegf in rats with acute myocardial ischemia.docxVIP

摘 要 实验目的：研究内关穴位注射香丹注射液预处理对大鼠急性心肌缺血炎性因子 C 反应蛋白（CRP）、肿瘤坏死因子ɑ（TNF-ɑ）、血管内皮生长因子（VEGF）的影响，为临 床防治心肌缺血损伤提供新的思路和方法。 实验方法: 本研究以SD大鼠为受试对象，心肌缺血前给予穴位注射香丹注射液预 处理14天，然后通过腹腔皮下注射异丙肾上腺素（ISO）造成心肌缺血模型，取心脏进 行心肌病理形态学观察，免疫组化法检测TNF-ɑ、VEGF的表达，取血清检测CRP的含量。 实验结果: （1）急性心肌缺血大鼠，其心肌具有典型的缺血缺氧的病理形态学改变。内关组 具有明显防治急性心肌缺血大鼠的心肌损害，减少病变范围，减轻病变程度，促进纤维 组织增生，加快修复的作用；药物组及曲池组对于心肌损害的改善不明显。内关组的上 述作用明显优于药物组及曲池组。 （2）CRP 在大鼠心肌缺血后表达增多，内关组、曲池组及药物组预处理后均可以 降低其表达，内关组的这一作用明显优于曲池组及药物组。 （3）TNF-α 在大鼠心肌缺血后表达增多，内关组、曲池组及药物组预处理后均可 以降低其表达，内关组的这一作用明显优于曲池组及药物组。 （4）VEGF 在大鼠心肌缺血损伤后表达有所增高，药物组和曲池组预处理后可以明 显增高其表达，与内关组比较有统计学意义。 实验结论: 内关穴位注射香丹注射液预处理对心肌缺血大鼠的心肌具有保护作 用。内关穴位注射香丹注射液预处理可以降低 CRP 和 TNF-α含量，增高 VEGF 的表达， 减轻心肌组织在急性心肌缺血中的损伤。内关穴位注射香丹注射液可以作为一种心肌缺 血的预处理手段，诱导心肌缺血耐受，减轻缺血后心肌组织的损伤。 关键词:内关 穴位注射 预处理 心肌缺血 CRP TNF-ɑ VEGF ABSTRACT Objective: To explore a new preventing and curing myocardial ischemia method by testing CRP(C reactive protein) 、 TNF-ɑ (tumor necrosis factor- ɑ ) and VEGF(vascular endothelial growth factor) on the rat model pre-injected XiangDanZhuSheYe into PC6. Methods: SD rats were received XiangDanZhuSheYe injection into PC6 for 14 days, following isoprenaline injection into belly cavity as to inducing myocardial ischemia model. Then the successful model rats were disposed. The myocardial tissue was observed for pathological changes. TNF-ɑ and VEGF expression were testing with immunohistochemical method. The content of CRP in serum was also tested. Result: (1) The typical pathological changes of myocardial ischemia and anoxia in model group can be seen. PC6 group could significiantly protect the myocardium tissue from ischemia and anoxia, narrow the area of pathological changes, reduce the degree of pathological changes, and promote fibroplasia, accelerate the eparation of the myocardial tissue. Drug control group and Quchi (LI11) group have less effect on protecting rats from myocardial ischemia. The method of pre-injection XiangDanZhuSheYe into PC6 was more effective than Drug control group and LI11 grou