三种褐藻中岩藻聚糖硫酸酯的纯化及结构研究-purification and structure study of fucoidan from three brown algae.docxVIP

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三种褐藻中岩藻聚糖硫酸酯的纯化及结构研究-purification and structure study of fucoidan from three brown algae.docx

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三种褐藻中岩藻聚糖硫酸酯的纯化及结构研究-purification and structure study of fucoidan from three brown algae

Ab Abstract Abstract Costaria costata. Saccarina japonica, Kjellmaniella crassifolia are three brown algae with high economic value. Fucoidan is a kind of heteropolysaccharides containing sulfate group, found in brown seaweeds and some echinoderms. Fucoidan shows various biological activities, which gradually become a reseach hotspot of the marine bioactive ingredients. In this article, 4, 3 and 4 fucoidan fractions were obtained by DEAE-Sepharose Fast Flow anion-exchange chromatography purification from three brown algae respectively. The contents of total saccharide, SO42-, and fucoidan of each fraction were determined, molecular structures of each fraction were also studied. The results and conclusions are as follows: Fucoidan was extracted from Costaria costata. Saccarina japonica, Kjellmaniella crassifolia by enzymatic method, named as CF, SF, KF respectively, and then purified by anion-exchange chromatography on DEAE-Sepharose Fast Flow. Four fractions, CF1, CF2, CF3 and CF4 were obtained from Costaria costata and the yields were 42.81%, 27.55%, 11.44% and 23.48% respectively. CF2 was a fraction with low SO 2- 4 content(1.01%), and CF4 was high with SO42- content(16.12%). Three fractions, SF1, SF2 and SF3 were obtained from Saccarina japonica and the yields were 12.95%、 2- 63.71% and 15.70% respectively. SF2 was a fraction with low SO4 content(5.56%), and SF3 was high with SO42- content(27.08%). Four fractions, KF1, KF2, KF3 and KF4 were obtained from Kjellmaniella crassifolia and the yields were 4.41%, 18.98%, 4.04% and 57.45% respectively. KF1 was a fraction with low SO42- content(4.27%), and KF4 was high with SO42- content(29.31%). The fractions from Saccarina japonica and Kjellmaniella crassifolia are pure, the relative molecular weights were estimated to be 48KD for CF1, 146KD for CF2, 155KD for CF3, 136KD for CF4, 136KD for SF1, 119KD for SF2 and 104KD for SF3 by Sephadex G-200 chromatography. The third fraction from Costaria costata by Sephadex G-200 c