球型脂联素对糖尿病大鼠胰岛素分泌和ir irs-1表达的影响-effects of globular adiponectin on insulin secretion and ir irs - 1 expression in diabetic rats.docxVIP

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球型脂联素对糖尿病大鼠胰岛素分泌和ir irs-1表达的影响-effects of globular adiponectin on insulin secretion and ir irs - 1 expression in diabetic rats.docx

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球型脂联素对糖尿病大鼠胰岛素分泌和ir irs-1表达的影响-effects of globular adiponectin on insulin secretion and ir irs - 1 expression in diabetic rats

Abstract II II Effects of globular adiponectin on insulin secretion and the expression of IR and IRS-1 in diabetic rats Abstract Objective To investigate the influence of globular adiponectin (gAd) on insulin secretion and the expression of IR (insulin receptor) and IRS-1 (insulin receptor substrate-1) in HFD/STZ induced diabetic rats. Methods Male Wistar rats were randomly divided into normal control group (NC group) and diabetic model group. Type 2 diabetic rats were induced by high-fat diet and low-dose STZ (28mg/kg) intraperitoneal injection. Then the diabetic model rats were further randomly divided into type 2 diabetic control rats (T2DM group) and type 2 diabetic rats treated with gAd (gAd group). Rats of gAd group were intraperitoneally injected with 3.5μg · kg-1· d-1 gAd for 7 days, while Rats of NC group and T2DM group received 0.9% saline instead. The concentrations of blood glucose, fasting serum insulin, cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), and high density lipoprotein (HDL-C) were measured. IR and IRS-1 protein expression were examined by western blotting and immunohistochemistry. IR and IRS-1 mRNA expression in liver and skeletal muscle tissue were analyzed by Quantitative Real-Time RT-PCR. Results (1) Serum blood glucose, cholesterol, triglyceride, and low density lipoprotein of gAd-treated group decreased than those of T2DM group, while fasting serum insulin of gAd-treated group increased than that of T2DM group. (2) IR protein and mRNA expression in hepatic and skeletal muscle tissues of T2DM rats increased compared to those of NC group. (3) Treatment with gAd reduced the expression of IR protein in hepatic and skeletal muscle tissues of diabetic rats, and it down-regulated IR mRNA expression in hepatic tissue. (4) Hepatic IRS-1 protein and mRNA expression of T2DM rats increased than those of NC group, but no statistical difference was found in skeletal muscle tissue between the two groups. (5)