水稻α-膨胀素基因osexpa8的功能分析-functional analysis of rice alpha-expansin gene os expla 8.docxVIP

重庆大学硕士学位 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT Rice roots consist of crown root and laterals. Growth angle, lateral root number, root diameter and the length enabled the roots form in different forms. Not only is the root the important organ of higher plants absorbing nutrients and moisture, but also carrying out the transformation of material synthesis, and assimilation. In addition, it is also the metabolic organ exchanging materials with the overground part. Thus, the roots play a crucial role in plant growth and development of plant, and in turn, the root status directly affects the nutrition absorption, plant growth and development as well as production levels. Expansin (EXP) is a kind of plant cell-wall loosening protein and plays a key role in the growth and development of plant. That is based on the acid growth theory of the plant cell wall. The EXP is a huge family in which α-expansin (EXPA) and β-expansin (EXPB) are two bigger subfamilies. There are at least 34 α-expansins in rice, but only 19 β-expansins. EXP proteins are generally consisted of 250-275 amino acids, whose similarity between different families is very low. Generally α-expansins were reported to work well on arrange of cell walls in dicot and non-commelinoid principally, but to have relatively low effect on grass cell walls. In contrast, β-expansins have more strong selectivity for grass walls and only marginal effect was found on dicot walls. In this paper, we investigated the functions of the root-specific α-expansin gene OsEXPA8 in rice, and the results are as follows: OsEXPA8-GFP fusion protein expression vector was constructed, and we analyzed the gene specificity of spatiotemporal expression as well as the responses to different stresses: ①The transient expression in onion epidermal cells indicated that OsEXPA8 localized on the cell wall ; ②Significant spatiotempora expression pattern of OsEXPA8 gene was revealed by quantitative real-time PCR, with 7-day-old root apices sho