水相合成cdte量子点对人宫颈癌细胞siha毒性分析-toxicity analysis of cdte quantum dots synthesized in aqueous phase to siha in human cervical cancer cells.docxVIP

StudiesoncytotoxicityofCdTequantumdotspreparedinaquousphaseusinghumancervicalcancerSiHacellsasamodelABSTRACTQuantumdots(QDs),alsoreferredtoassemiconductornanocrystals,havedrawnincreasingattentioninbiologicalandmedicalfieldsduetotheirsize-dependentandexcellentopticalpropertiessuchashighphotoluminescent(PL)quantumyield,narrowemissionprofiles,broadexcitationspectra,andgoodbiocompatibility.ButthetoxicityofQDsremainedanoutstandingdisadvantage.Inthisstudy,thecytotoxicityofCdTeQDswasevaluatedusinghumancervicalcancerSiHacellsasamodelwithWST-1viabilityassaysystematicallyconsideringtheirsizes,concentration,stablizers,exposuretimeanddifferentsurfacemodifications.Firstly,wepreparedCdTeQDswithdifferentemissionwavelengthusingaqueoussynthesisapproach,andinvestigatedthecytotoxicityoftheQDsviaWST-1cellviabilityassaywithSiHacells.OurresultsrevealedthatCdTeQDswithglutathione(GSH)asastabilizerwaslesstoxiccomparedtoCdTeQDswith3-mercaptopropionicacid(MPA),dimercaptosuccinicacid(DMSA)andN-acetyl-L-cysteine(NAC).WealsoobservedthatthecellviabilitysignificantlydecreasedwithsmallersizeofQDs,higherexposureconcentrationandlongerexposuretime.Accordingtoourcalculation,thesufacestatemightcontributealottothecytotoxicityofCdTeQDs.Secondly,surfacemodificationsofCdTeQDswereconductedusingbovineserumalbumin(BSA),denaturedbovineserumalbumin(dBSA)andthiolpolyethyleneglycerol(PEG).WefoundthatBSA-coatedQDsanddBSA-coatedQDsshowednoreductionincytotoxicity,butthePEG-coatedQDsshowedlesscytotoxicitycomparedtotheQDscore.AfterthecoatingofCdSandZnSshell,thecytotoxicityofQDsdecreasedremarkably.Littlereductionofcellviabilitywasobservedafter24hoursofCdTe/CdS/ZnSQDsexposure,andcellviabilitywas89.3%after48hours.FurtherinvestigationrevealedthatthereleaseofCd2+fromtheCdTecoredecreasedremarkablyafterthecoatingofCdSandZnSshell,whichsuggestedthepassivationshellofZnSpreventedthereleaseofCd2+effectively,andoptimizedthesurfacestateofCdTeQDs.KEYWORDS:CdTeQDs;SiHacells;cytotoxicity;surfacemodification目录第一章绪论......................