山东省大豆疫霉的鉴定及生物学特性分析-identification and biological characteristics analysis of phytophthora sojae in shandong province.docxVIP

AbstractPhytophthorasojaeKaufmannetGerdemannisoneofthemostimportantplantpathogenicfunguswithstrongparasitesandenormouseconomicdamageonsoybean.BasedontheisolationofthecausalorganismofsoybeanphytophthorarootrotinShandongprovince,identificationofPhytophthorasojaeusethemethodofmorphologyandpolyacrylamidegelelectrophoresisofsolubleproteins,sequencingofribosomalDNA-ITSofPhytophthorasojae,effectsofmediumnutrientontheattachmentmodeofantheridiaonoogoniaandonmycelialgrowthrateofPhytophthorasojaewerestudiedsystematicallyinthisdissertation.Themainresultswereasfollows.IsolationanddistributionofP.sojaeinShandongprovinceThe225soilsamplesinsoybeanfieldwerecollectedfromthemainsoybeanproducingareasinShandongprovince,andPhytophthorasojaewasdetectedbythemethodofsoybeanleaf-discbaiting.TheresultindicatedthatP.sojaehasbeenbaitedfrom16soilsamplescollectedin6counties,includingshouguang,rizhao,junan,feixian,zouchengandjining.Atotalof5isolatesofP.sojaewereobtainedfromsoilsamples.However,P.sojaehasnotbeendetectedfrom81soilsamplescollectedin9cities,includingyantai,qingdao,binzhou,zibo,dezhou,jinan,taian,liaochengandheze.IdentificationofPhytophthorasojaeusethemethodofmorphologyandpolyacrylamidegelelectrophoresisofsolubleproteinsFiveisolatesofPhytophthora,separatedfromsoybeanfieldinShandongprovince,wereidentifiedusethemethodofmorphologyandpolyacrylamidegelelectrophoresisofsolubleproteins.TheresultsindicatedthatallofthefivetestedisolateswerePhytophthora,themycelialgrowthratewasslowonlimabeanagar(LBA)media,hyphaeclosely,nonseptate,abundant，roundedhyphalswellingsinchains,right-angleandshrinkinginbranch.Sporangiumwasinverted-pear-shaped,non-papillate,internalproliferationofsporangiophore,zoosporesformedwithinsporangium,homothallism,roundedarchegoniumandparagynousantheridium.Electrophoresispatternamongthefivetestedisolatesandstandardstrainwerefundamentalidentical.Therewere13mutualmainbandsatthemobilityof0.08,0.09,0.11,0.13,0.15,0.18,0.28,0.30,0.32,0.34,0.45,0.52and0.57amongfivetestedisolatesandstanda