水处理工艺中氟喹诺酮类物质分布的检测分析-detection and analysis of fluoroquinolones distribution in water treatment process.docxVIP

关键词：水处理工艺；氟喹诺酮；固相萃取；HPLC；LC-MS/MS(Q-TOF)AbstractFluoroquinoloneantibiotics(FQs)isoneofthemostwidelyusedantibioticsusedinhumanmedicine,agriculture,animalhusbandryandaquaculture.Inrecentyears,theproblemofFQsresidueinwaterandit’spotentialhazardsattractedgreatattentionoftherelevantdepartmentsandresearchers,ItisreportedthattheFQsweredetectedinsuorcewaterandtapwater,hence,itisofgreatsignificancetoestablishanefficientandaccuratemethodstodetecttheFQsscontentinsourcewaterandthewatertreatmentprocess,andtostudytheFQsabatementlawinwatertreatmentprocesstoguaranteethesafetyofdrinkingwater.FiveFQs—pipemidicacid(PIP),norfloxacin(NOR),ciprofloxacin(CIP),enrofloxacin(ENR)andofloxacin(OFL)werechoosedastargetedcontaminant.Allthesamplesformsourcewaterandwatertreatmentprocesswereenrichedbysolidphaseextraction(SPE),andweredetectedbyhighperformanceliquidchromatography(HPLC)andquadrupoleconnectflighttimemassspectrometry(LC-MS/MS(Q-TOF)).FinallyanefficientdetectionmethodswasconstructedtosimultaneousdetectresiduelevelsoffivekindsofFQsindrinkingwatertreatmentprocess,andthentheremovalefficiencyofthewatertreatmentprocesswassystematicallyevaluated.ThesampleswaspretreatedbySPE.Fristtheextractvolume,thecolumnflowrateofeluent,eluentandelutionvolumeofextractionweregraduallyoptimizedtoachievethehighdemandofhighefficiencyandpurificationeffect.Inthisstudy,apreliminarydetectionmethodoffiveFQswereconductedwithHPLC,themethodsatisfytheconditionofbeautifulpeakshapes,clearseparation,fastandaccurateliquidtesting.TheLC-MS/MS(Q-TOF)conditionswasoptimizedandeventuallyestablishedbasedontheHPLCdetectconditions.FivesubstancescansimultaneouslydetectedandaccuratequantitativedeterminationwiththemethodwhichrealizetheaccuratequantificationofthetraceamountsofFQs.Asolidphaseextraction(SPE)-liquidchromatography-massspectrometry(LC-MS/MS(Q-TOF))ofthesamplehandlinganddetectionmethodwasestablished.TherecoveriesofFQsinSonghuaRiversamplesspikedwithFQsstandardsolution(100ng/L)arebetween81%and108%,andtherelativestandarddeviation(R