生态因子与三七稳定同位素比率的关系分析-analysis of the relationship between ecological factors and stable isotope ratio of panax notoginseng.docxVIP
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生态因子与三七稳定同位素比率的关系分析-analysis of the relationship between ecological factors and stable isotope ratio of panax notoginseng
生态
生态因子与三七稳定同位素比率的关系研究
PAGE
PAGE 10
摘要
三七是我国为数不多的对气候环境条件要求十分严格的中药材品种,虽然栽 培历史上曾有 10 余个省区引种,但几乎都没有成功 ,“道地药材”如果离开了 它原有的生长环境,即使能够生长,长成后其外形虽然没有多大明显变化,但其 内在的有效含量即特有药效要比原来大大降低。中药材指纹技术在中药材道地性 研究中起着重要的作用,尽管不同产地的同种药材上述各种指纹图谱有明显的差 异,但将其用于产地溯源仍存在不确定性,而以往的指纹图谱主要从分子(有效 成分、DNA)角度进行的,而同位素指纹图谱是从原子的角度确定中药材产地 和种质资源特性。本项目采用相关分析、逐步回归分析以及通径分析对云南、广 西、广东等三七主产区进行生态因子对三七不同部位稳定同位素比率的影响的研 究,确定影响同位素比率变化的主导生态因子及其权重,为三七产地溯源提供技 术支撑。研究结果表明:
1. 生态因子对三七各部分稳定碳同位素比率的回归分析表明,茎叶、块根、 芦头、筋条、须根中稳定碳同位素比率与土壤性质、土壤养分元素、地理气候因 子都有显著的相关性。
2. 生态因子对三七各部分稳定氮同位素比率的回归分析表明,三七茎叶、 块根、芦头、筋条、须根中稳定氮同位素比率与土壤性质、土壤养分元素、地理 气候因子都有显著的相关性。
3. 生态因子对三七各部分稳定氢同位素比率的回归分析表明,只有芦头和 须根中稳定氢同位素比率与地理气候因子有显著的相关性。
4. 生态因子对三七各部分稳定氧同位素比率的回归分析表明,三七茎叶、 块根、筋条中稳定氧同位素比率与地理气候因子有显著的相关性。
5.三七茎叶、块根中稳定同位素与土壤性质、土壤养分元素、地理气候因子 逐步回归和通径分析得出,对茎叶和块根稳定碳同位素比率影响效应最大的都是 纬度;对茎叶稳定氮同位素比率影响效应最大的是有效 Fe;对块根稳定氮同位 素比率影响效应最大的是七月最高温;对茎叶稳定氧同位素比率影响效应最大的 是湿度;对块根稳定氧同位素比率影响效应最大的是纬度。
关键词:三七;稳定碳同位素比率;稳定氮同位素比率;稳定氢同位素比率; 稳定氧同位素比率;生态因子
Study on the relationship between the ecological factors and stable isotope ratios in Panax notoginseng
Ding Ying (Soil Science)
Directed by Professor He Zhongjun
ABSTRACT
Panax notoginseng is one of the few Chinese herbal medicines varieties which is very stringent requirements in Climatic and environmental conditions, although there have been more than 10 provinces to plant it in the history of cultivation, almost no successful. If the Geoherbs left its original growth environment, even though it can grow and its shape is not much significant change after grow into, its intrinsic effective content is greatly reduced than the original. Chinese herbal medicines fingerprint technology plays an important role in the authentic Chinese herbal medicines research, despite there are significant differences in the same herbs fingerprint of the different origins, there are still uncertainties using in origin traceability, the previous fingerprint conducted by the molecule (the active ingredient, DNA), but isotope fingerprint determine the characteristics of Chin
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