活化后从胞质转位至胞核通过识别和结合基因启动子区的顺式作用.PPTVIP

Company Logo LOGO AtNAP, a NAC family trancription factor,has an important role in leaf senescence 学术报告—文献阅读汇报 Company Logo Outline Introduction 1 2 Result and Conclusion 3 Discussion 4 Experiment content Introduction 1.1 Leaf Senescence Transcription Factors TF 能够结合在某基因上游特异核苷酸序列上的蛋白质，活化后从胞质转位至胞核，通过识别和结合基因启动子区的顺式作用元件,启动和调控基因表达。 1.2 NAC family transcription factor Introduction WRKY DREB bZIP NAC MYB NAC 含有NAC结构域的蛋白统称为NAC蛋白 植物生长发育 激素调节 环境胁迫应答 NAM CUC2 ATAF1/2 Experiment content Inducible overexp- ression of AtNAP AtNAP expression in T-DNA line Subcellular location of AtNAP AtNAP expression during leaf senescence 3.1 AtNAP is upregulated during leaf sencence in Arabidopsis Results and Conclusion 3.2 AtNAP is targeted to nuclei Results and Conclusion 3.3 The AtNAP expression is knocked out in one T-DNA line and knocked down in another line Results and Conclusion 3.4 Leaf senescence is significantly delayed in the atnap null mutant plants Results and Conclusion Results and Conclusion Results and Conclusion 3.5 AtNAP homologs in rice and kidney bean are specifically expressed in senescing leaves Results and Conclusion Results and Conclusion Company Logo 3.6 AtNAP restores the atnap null mutant plants to wild type Results and Conclusion 3.7 Inducible overexpression of AtNAP causes precious senescence Results and Conclusion Results and Conclusion 3.8 Microarray analysis of the expression profile of AtNAP Results and Conclusion RT-PCR RNA gel blot T-DNA insertions Plasmid construction Plant transformation Complementation test Disscussion 1.冶晓芳,唐益苗,高世庆,等.植物NAC转录因子的研究进展. 生物计数通报,2009,10(6)20–24. 2.李鹏,黄耿青,李学宝.植物NAC转录因子. 植物生理与分子生物学,2010,46(3)294–300. 3.郭玉娟,魏强.高羊茅中衰老相关转录因子AtNAP同源基因的克隆及功能分析. 复旦学报,2010,49(5)544–558. 4.彭辉,于兴旺,成慧颖,等.植物NAC转录因子家族研究概况. 植物学报,2011,45(2)236–248.报,2011,45(2)236–248. 5.史红飞,高翔,陈其皎, 等.小麦NAC转录因子的基因克隆与序列分析. 西北农林科技大学学报,2011,31