内皮祖细胞的研究进展ppt课件.pptVIP

;Definition They are circulating, bone marrow-derived cells that are functionally and phenotypically distinct from mature endothelial cells They can differentiate into endothelial cells in vitro, as assessed by expression profiles and functional characteristics They can contribute to in vivo vasculogenesis and / or vascular homeostasis;Embryonic EPC;Adult EPC;Origin and differentiation of endothelial progenitor cells;EPCs’ Development; VEGFR and VEGF Receptor VEGFR-1 (flt-1) VEGFR-2 (flk-1/KDR) VEGFR-3 (flt-4) Ligand VEGF、 VEGF-B, C, D, E; VEGFR and VEGF; Tie receptor and ligand：受体酪氨酸激酶家族 receptor Tie-1 (Tie) Tie-2 (Tek) Tie-2 ligand：Angiopoietin (Ang，血管生成素 ) Ang-1：血管内皮化 Ang-2：血管周围细胞和内皮细胞分离; Tie receptor and ligand：受体酪氨酸激酶家族 Tie-2缺陷的胚胎不能建立血管结构的完整性 Ang-1缺陷鼠也表现为血管生成缺陷 ; Ephs family Erythropoietin producing hepatocyte receptor (Eph) Ligand: ephrins (Eph A、B) Eph B可调节 Ang-1 和 Tie-2的表达;CD34+, Flk-1+;CD34-selected culture-dish non-adherent putative EPC (CDNAC) change to a more mature endothelial phenotype during differentiation while the progenitor phenotype remains stable and the monocytic phenotype decreases. ;CD34-selected putative EPC from culture-dish adherent cells (CDAC) express lower levels of endothelial and progenitor markers than CDNAC. ;VEGFR-2/Flk-1+; CD34+/ VEGFR-2+ cells can behave as EPCs. CD133+/ CD34+/ VEGFR-2+ cells represent a more primitive EPC.;Putative EPCs take up acetylated low density lipoprotein (Ac-LDL) and bind the endothelial specific lectin UEA-1.;Mobilization of EPCs;Mobilization of EPCs;Chemotaxis, Migration and Invasion SDF-1 VEGF ; EPCs were originally thought to be present only during embryonic development. Evidence accumulating over several years suggests that they can persist in adult life. This has generated interest in the use of EPCs for neovascularization of ischemic or injured tissue and for the clinical assessment of risk factors for various disea