脊髓损伤后微管稳ppt课件.ppt

脊髓损伤后微管稳定减少瘢痕并引起轴突再生Microtubule Stabilization Reduces Scarring and Causes Axon Regeneration After Spinal Cord Injury;概述;概述;紫杉醇;;脊髓损伤后Taxol治疗能否减少瘢痕形成？;Fig. 1 Taxol decreases scarring induced by spinal cord injury;Figure S1: Taxol decreases fibronectin, NG2 proteoglycan and collagen IV accumulation induced by spinal cord injury ;Figure S2: Taxol does not interfere with proliferation;Figure S3: Taxol does not induce apoptosis in the injured spinal cord;脊髓损伤后Taxol治疗能否减少瘢痕形成？;稳定微管网能否阻碍TGF-β信号从而减弱纤维形成？;Fig. 2 Taxol dampens TGF-b signaling;Figure S4: Taxol sequesters Smad2 onto microtubules;(G and H)在培养的星形胶质细胞中，Taxol中和了TGF-β诱导的Smad2/3核转运（箭的头）引起细胞质Smad2/3聚集（箭头） (I)损伤7天后，Taxol治疗干扰了SCI诱导的磷酸化Smad2/3核转运. BSA, bovine serum albumin; DAPI, 4′,6′-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide二甲基亚砜;稳定微管网能否阻碍TGF-β信号从而减弱纤维形成？;Taxol是否在SCI后降低CSPGs？;;;Figure S5: Taxol promotes axonal outgrowth of primary neurons plated on inhibitory substrates;Taxol治疗损伤部位能否允许在体轴突再生?;Taxol促进轴突再生和功能恢复。;;Figure S6: Taxol promotes formation of growth cone-like structures at the tip of the 5HT-positive axons;Taxol治疗是否引起中等脊髓挫伤后的功能恢复?;讨论;;;Taxol 在体治疗;TGF-β;;Taxol dampens TGF-b signaling;CTB;AAV简介;AAV简介;AAV选择的条件;AAV病毒的感染细胞;RNA原位杂交;;探针的选择;探针的标记;病毒的加样量