布氏杆菌病ppt课件_2.pptVIP

BRUCELLOSIS Introduction History Brucellosis: Disease of domestic and wild animals (zoonosis): Transmittable to humans. It has different non-specific symptoms and signs “No disease, not excepting tuberculosis and syphilis, is more protean in its manifestations”..(Simpson). Marston – Surgeon serving with Royal Artillery during Crimean war-reported first accurate description of human brucellosis. 1886, Bruce isolated Micrococcus (later Brucella) Melitensis from spleens of malta fever victims. Introduction and History (Cont’d.) Zamit – maltese physician, identified native goats as infection reservoir, during his work with Mediterranean fever commission 1904-1907. He also identified fresh goat’s milk as vehicle of transmitting disease from animals to humans. 1895, Bang (Danish Veterinarian) identified Bacillus (later Brucella) abortus as cause of contagious abortions in cattle. Evans (American bacteriologist) recognized relation of Malta fever agent and Bang’s disease Introduction and History (Cont’d.) Genus – Bacillus, micrococcus, causing Malta fever, renamed Brucella, to honor Bruce. Traum, 1914, isolated Brucella Canis, from aborted Kennel-bred dogs. Carmichael, 1966, isolated Brucella Canis, from aborted Kennel-bred dogs. Brucella ovis from sheep and Brucella neotomae from desert wood rats, added to Brucella species, but to date these two not shown to cause human infections. Introduction and History (cont’d.) 1994, British and American workers, independ- ently, isolated previuously unknown Brucella organism from carcasses of marine mammals and cetaceans on Scotland coast, and from dolphin in California. These isolates-homogenous with distinctive metabolic, sensitivities to dye, phage sensitivity-tentatively named Brucella Maris. THE PATHOGEN Brucellae: Small, GN cocobacilli, non-motile, non-spore forming. Brucellae grow aerobically. Some spp. Require supplemental carbon dioxide for primary isolation. Any high-quality peptone-based