小鼠放创复合伤伤口愈合延迟机理分析-mechanism analysis of delayed wound healing in mice with combined radiation and wound injuries.docx

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2018-08-07 发布于上海
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小鼠放创复合伤伤口愈合延迟机理分析-mechanism analysis of delayed wound healing in mice with combined radiation and wound injuries.docx

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小鼠放创复合伤伤口愈合延迟机理分析-mechanism analysis of delayed wound healing in mice with combined radiation and wound injuries

ABSTRACTCombinedradiation-traumainjuryaremainlyobservedinradiationtreatmentofcancerandradiationinjurywithtraumaticpatients.Thesignificantissueisdelayedwoundhealing.Existingresearchshowsthat:delayedwoundhealingcausedbytotalbodyirradiationinjuryistheresultofmulti-healing-factorcooperationwithreductionandfunctionalimpairmentofhematopoieticcellsandrepaircells.Thechangesofthedamagemaybecloselyrelatedtoinjuryseverity,radiationdosage,individualdifferencesandotherfactors.Itsmechanismisnotclearatpresent.Cytokinesareaclassofsmallmoleculepeptidewhichhavesignificantregulatoryfunctionsincellgrowthanddifferentiation.Theyareimportantsignaltransmittersbetweencellandtheextracellularmatrix.VEGF(VascularEndothelialGrowthFactor),PDGF(Platelet-derivedGrowthFactor),TNF-α(TumorNecrosisFactor)areimportantmessengermoleculesandorganizersinwoundhealing.Studieshavefoundthatindifferentperiodsofcombinedradiation-traumainjury,PDGF,VEGFandTNF-αexpressiondecreasedsignificantly.Theexistingresearchesincombinedradiation-traumainjuryrepairwerefocusedonfunctionofcytokinesintheprocessofwoundhealing.Althoughmorecomplexanimalmodelsofinjurywerereportedintheliterature,awarenesswasquitedifferentinmanyfieldsasradiationdosage,animalmortalitystatisticsandlevelofdelayedwoundhealing.Becausethereisnoexclusivestandardmodel,weconstructahighstable,goodrepeatability,standardizedanimalmodelofcombinedradiationinjury,analyzethefunctionandsignificanceofPDGF,VEGFandTNF-αinwoundhealingofcombinedradiationinjury,andexploretherelationshipbetweencytokinesandinflammatorycells.Itisnecessaryforthestudyofmechanismoftotalbodyirradiationinwoundhealing.ObjectiveBuildmodelswithdifferentdosesoftotalbodyradiationwithskindefect/incisedwoundbymice.Testtheindexeswhichcanreflectthelevelofinjuryasinflammatorycells,woundrepaircellsandnewbloodcapillaries,andtheexpressionofPDGF、VEGF、TNF-αinwoundsofcombinedradiationinjury(Within14daysafterinjury),studythedifferentdosagesoftotalbodyradiationeffectsonskin,explorethefunctionofPDGF,VEGFandTNF-αinwound