小鼠精原细胞分离培养及其影响因素的实验分析-isolation and culture of mouse spermatogonia and experimental analysis of its influencing factors.docx

七卵泡刺激素对体外培养的精原细胞的影响……中文摘要·······························································……英文摘要·······························································……·····································································……前言材料与方法····························································……·····································································结果……·····································································讨论参考文献·······························································……八高氧对体外培养的精原细胞增殖和凋亡的影响……中文摘要·······························································……英文摘要·······························································……·····································································……前言材料与方法····························································……·····································································结果……·····································································……讨论参考文献·······························································……·····································································……致谢攻读学位期间发表的学术论文目录······························矛山东大学博士学位论文、小鼠精原细胞分离培养及其影响因素的实验研究博士研究生张晓丽导师高英茂专业人体解剖与组织胚胎学，、中文摘要，据报道近年来男性不育的发生率有所升高无精少精和弱精的患者，，有所增加因而对精子发生机制及其影响因素的研究也就成了生殖医学领域的一大研究热点’精子发生从精原细胞开始。，，、、、口、一经精母细胞和精子细胞形，成成熟的精子其间经历多次有丝分裂和两次减数分裂受神经内分泌，旁分泌及多种细胞因子的调节匙夕巡墅建弊物遨缪一队通多二复’‘口。越哩吕基影鲤素的研究是预咐喧疽男胜不育和研制男性节育方法的基础性研究一、、、碑产气、尹目前这方面的研究多为在体研究体外研究较少在体研究结果显，。，、、。示导致男性精子数目及质量下降的因素主要有遗传缺陷慢性疾患过度、劳累和精神负担环境污染不良的饮食和生活习惯等件，由于在体研究受、，神经体液等许多复杂因素的影响因而在体外培养条件下研究生精过程及。其影响因素便成了必然趋势，，最原始的一种精原细胞即型精原细胞是生精干细胞通过非对称性有，，丝分裂这种细胞部分分化为型精原细胞另一部分则仍然保持为生精干。，细胞】在进行体外研究生精过程的机制及其影响因素时最先遇到的一个。、、问题就是如何提取和纯化培养生精干细胞本项研究以小鼠为实验动物首、、、、。先研究了生精干细胞的提取，纯化培养的最佳方法然后检测了温度、重组卵泡刺激素高氧等对生精干细胞存活代谢增殖凋亡等的影响一精原细胞的分离和纯化培养，，精原细胞中的型精原细胞是一种生精干细胞可通过非对称性细胞分。。裂分化为型精原细胞并进行自我复制目前尚无法提取和纯化这种精原，干细胞只能提取和纯化培养含有生精干细胞的全部精原细胞本实验筛选一一、、，出了分离培养和体外扩增精原细胞的一种比较好的方法为生精过程及一其侧一月一一一一一、一、、。影响因素的体外研究提供了重要的技术支持一一、一一、一山东大学博士学位论文，，。精原细胞在辜丸中的含量很少每护个皋丸组织细胞中仅含有一个，精原细胞因此要获得足量的精原细胞有一定的难度