小鼠前列腺癌原位模型建立及kng在前列腺癌发病中作用-establishment of mouse prostate cancer in situ model and the role of kng in the pathogenesis of prostate cancer.docx

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2018-08-07 发布于上海
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小鼠前列腺癌原位模型建立及kng在前列腺癌发病中作用-establishment of mouse prostate cancer in situ model and the role of kng in the pathogenesis of prostate cancer.docx

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小鼠前列腺癌原位模型建立及kng在前列腺癌发病中作用-establishment of mouse prostate cancer in situ model and the role of kng in the pathogenesis of prostate cancer

小鼠前列腺癌原位模型的建立及Kng在前列腺癌发病中的作用英文摘要EstablishmentoftheprostatecancerorthotopicmodelinmiceandtheroleofKnginprostatecancerAbstractPart1EstablishmentoftheprostatecancerorthotopicmodelinmiceObjectiveToestablishaprostatecancerorthotopicmodelinmiceanddiscussitsvalueofapplication.MethodsHistologicallyintacttumortissuesharvestedfromasubcutaneousprostatetumorofC57BL6syngenicmousewerecutintosmallpiecesandtransplantedintomicesubcutaneous(subcutaneousgroup,10)andprostategland(orthotopicgroup,30).Therateofmetastasisandthesurvivaltimeofthesetwomodelswerecompared.ResultsTherateoftumorformationofsubcutaneousgroupandorthotopicgroupwas100%.Thesurvivaltimeoforthotopicmodelwas(14.6±2.6d)days,markedlyshorterthanthatofsubcutaneousmodel(39.8±4.7d)days(P0.01).Therateofmetastasisoforthotopicgroupmicewasmorehighthanthemiceofsubcutaneousgroup(73.3%vs0,P0.01).ConclusionTheprostatecancerorthotopicmodelisastable,highrateofmetastasistodistalorganmodel,itisanoptimalclinicalanimalmodeltoobservemetastasis.Part2TheroleofKnginprostatecancerObjectiveToexploretheinhibitoryeffectofKngonmetastasisofprostatecancer.MethodsThekininogenproteinandkininogenmRNAinprostatecancerwereconfirmedbyWestern-blottingandRT-PCR.UsingfifteenKng-/-miceandKng+/+mice,theprostatecancerorthotopicmodelwereestablished.Therateofmetastasisandthesurvivaltimeofthesetwomodelswerecompared.ResultsThekininogenproteinandkininogenmRNAinmiceprostatecancerweremorehighthanmiceprostate.Infiftydays,12Kng-/-miceweredied(12/15)and15Kng+/+miceweredied(15/15).TherateofmetastasisofKng-/-micewasmarkedlylowerthantheKng+/+mice(20%vs66.7%,P0.05).Conclusion1.ThelevelofkininogenproteinandkininogenmRNAishighinprostatecancer.2.Thekininogengeneknockoutcanprolongsurvivaltimeofprostatecancer.3.Thekininogengeneknockoutcanreducetherateofmetastasisofprostatecancer.英文摘要小鼠前列腺癌原位模型的建立及Kng在前列腺癌发病中的作用Keyword:prostatecancer,animalmodels,kallikrein-kininsystem,kininogenWrittenby:LiuGuodaoSuoervisedby:WenDuangaiChenWeiguo目录前言1第一部分小鼠前列腺癌原位移植模型的建立4材料和方法4一、实验材料4结果8讨论11结论13参考文献13