B细胞血液肿瘤患者外周血CD40CD154表达及意义.doc

B细胞血液肿瘤患者外周血CD40CD154表达及意义 　　[摘要] 目的 分析B细胞血液肿瘤外周血淋巴细胞CD40、CD154表达率的特点。方法 28例B细胞血液肿瘤患者分为多发性骨髓瘤（Multiple Myeloma，MM）17例，B细胞性非霍奇金淋巴瘤（B-NHL）11例；选取30例健康体检人员作为对照组。应用流式细胞术检测B细胞血液肿瘤患者外周血淋巴细胞（Peripheral Blood Lymphocytes，PBL）CD40、CD154的表达率。结果①MM组：CD40表达率为（17.50±8.32）%，CD154表达率为（1.00±3.26）%；B-NHL组：CD40表达率为（29.84±15.61）%，CD154表达率为（2.01±0.59）%；对照组：CD40表达率为（6.48±3.34）%，CD154表达率为（3.25±1.04）%。②化疗后B-NHL患者外周血淋巴细胞CD40表达低于化疗前，P0.05。③MM患者III期组PBLCD40表达率高于I～II期组，P0.05。B-NHL患者PBL CD154表达率III～IV期低于I～II期组，P0.05。 结论 ①MM患者、B-NHL患者均存在PBL CD40高表达，B-NHL患者同时存在PBL CD154低表达；CD40高表达提示MM预后差，CD154低表达与B-NHL预后有关。②化疗降低了B-NHL患者PBL CD40表达率。为临床上进行B细胞血液肿瘤诊断与治疗提供一定的依据。 　　[关键词] B细胞；血液肿瘤；CD40；CD154 　　[中图分类号] R732 [文献标识码] A [文章编号] 1674-0742（2014）08（b）-0009-03 　　[Abstract] Objective To analyze the characteristics of expression rate of peripheral blood lymphocytes （PBL） CD40 and CD154 in patients with B-cell hematologic malignancies. Methods 28 cases of patients with B-cell hematologic malignancies were divided into 17 cases with multiple myelom（MM） and 11 cases with B-cell non-Hodgkins lymphoma（B-NHL）； 30 healthy people underwent healthy examination were selected as the control group. Flow cytometry was used to detect the expression rate of PBL CD40 and CD154 of patients with B-cell hematologic malignancies. Results 1. MM group： the expression rate of CD40 was （17.50 ± 8.32）%， and that of CD154 was （1.00 ± 3.26）%； B-NHL group： the expression rate of CD40 was （29.84 ± 15.61）%， and that of CD154 was （2.01 ± 0.59）%； Control group： the expression rate of CD40 was （6.48 ± 3.34）%， and that of CD154 was （3.25 ± 1.04）%. 2.The expression rate of PBL CD40 of B-NHL patients after chemotherapy was lower than that before chemotherapy， P0.05. 3.PBL CD40 expression rate of stage III subgroup in MM group was higher than that of stage I and stage II subgroups， P0.05. PBL CD154 expression rate of stage III and IV subgroups in B-NHL group was lower than that of stage I and II subgroup， P0.05. Conclusion 1. Patients with MM an