68岁儿童龋病相关唾液蛋白组电喷雾离子肼―串联质谱分析.docVIP

68岁儿童龋病相关唾液蛋白组电喷雾离子肼―串联质谱分析 　　[文章编号] 1000-1182(2014)03-0297-06 　　[摘要] 目的 采用电喷雾离子肼―串联质谱分析法（ESI-MS/MS）比较高龋组和无龋组儿童唾液蛋白组，初步 　　探索唾液蛋白组与龋病的关系，并探索龋敏感性相关的生物标志物。方法 以高龋组、无龋组儿童各10名为研究对象，收集唾液样本，进行聚丙烯酰胺凝胶电泳（SDS），经过滤-辅助的蛋白酶解（FASP）及液相柱色谱分析后，进行ESI-MS/MS鉴定，并对高龋唾液组和无龋唾液组组间的蛋白差异进行分析。结果 高龋组蛋白量显著 　　高于无龋组，高龋组、无龋组鉴定的多肽数分别为602、481个，分别属于286、227个蛋白。两组之间差异表达多 　　肽数为361个，差异表达蛋白数为118个，包含基质金属蛋白酶9、黏蛋白7、乳铁蛋白、碳酸酐酶6、天青杀素、冷凝集素等。结论 高龋组儿童唾液蛋白量高于无龋组，基质金属蛋白酶9、黏蛋白7、乳铁蛋白、碳酸酐酶6、天青杀素、冷凝集素等高龋组与无龋组之间差异表达蛋白的检测，为进一步探索龋敏感性相关生物蛋白标志物奠定了 　　基础。 　　[关键词] 龋病；唾液蛋白组；电喷雾离子肼―串联质谱；生物标志物 　　[中图分类号] R 781.1 [文献标志码] A [doi] 10.7518/hxkq.2014.03.020 　　 　　Relationship between dental caries and salivary proteome by electrospray ionization ion-trap tandem mass spectro-metry in children aged 6 to 8 years Yan Guowei1,2, Huang Wenming1,2, Xue Honglei1,2, Jia Yuhuan1,2, Yang Deqin1,2. (1. Dept. of Endodontics, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400017, China; 2. Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing 400017, China) 　　[Abstract] Objective To explore the relationship between salivary proteome and dental caries and to promote the bio-marker studies of dental caries susceptibility by comparing the salivary proteome of caries-active children and caries-free children with electrospray ionization ion-trap tandem mass spectrometry (ESI-MS/MS). Methods Ten caries-active children and ten caries-free children were sampled. The salivary proteome of the two groups was studied, and the differential protein between the two groups was analyzed by ESI-MS/MS after sodium dodecyl sulfate polyacrylamide gel electrophoresis, filter-aided sample preparation, and liquid chromatography. Results The concentration of salivary protein was higher in the caries-active group than in the caries-free group. The polypeptide counts of thecaries-active and caries-free groups were 602 and 481, which belonged to 286 and 227 proteins, respectively. The differential polypeptide count