15―LOX―1 对LPS作用下肺动脉内皮细胞增殖和炎性因子影响.docVIP

15―LOX―1 对LPS作用下肺动脉内皮细胞增殖和炎性因子的影响 　　[摘要] 目的 探讨下调15-脂氧酶-1（15-LOX-1）对脂多糖（LPS）作用下肺动脉内皮细胞（PAECs）和炎性因子分泌的影响。 方法 体外培养PAECs，并随机分为4组：对照组、LPS组、15-LOX-1 siRNA对照组（scramble组）和15-LOX-1 siRNA组。采用Real-time PCR检测15-LOX-1表达，MTT法检测PAECs增殖变化，ELISA法检测产物13-HODE以及炎性因子白细胞介素1α（IL-1α）和白细胞介素6（IL-6）的变化。 结果 与LPS组比较，15-LOX-1 siRNA组15-LOX-1 mRNA表达降低，PAECs增殖增多，13-HODE、IL-1α和IL-6分泌减少，差异有统计学意义（P 0.05）。 结论 下调15-LOX-1可抑制炎性因子分泌，促进PAEC增殖，改善LPS对内皮的损伤。 　　[关键词] 肺动脉内皮细胞；15-脂氧酶-1；脂多糖；炎性因子 　　[中图分类号] R563.9 [文献标识码] A [文章编号] 1673-7210（2017）11（a）-0024-03 　　The effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells and inflammatory cytokines by LPS 　　LIU Ye XING Wenjing LI Sijia 　　Department of Immunology， Harbin Medical University， Heilongjiang Province， Harbin 150081， China 　　[Abstract] Objective To explore the effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells （PAECs） and inflammatory cytokines by LPS. Methods PAECs were cultured in vitro， and divided into 4 groups， including control group， LPS group， 15-LOX-1 siRNA scramble group （scramble group） and 15-LOX-1 siRNA group. The expression of 15-LOX-1 mRNA was detected by Real-time PCR. The proliferation of PAECs was examined by MTT. The expressions of 13-HODE， IL-1α and IL-6 were detected by ELISA. Results In the 15-LOX-1 siRNA group， the expression of 15-LOX-1 mRNA was reduced， the proliferation of PAECs was increased， and the secretions of 13-HODE， IL-1α and IL-6 were reduced compared with the LPS group with statistically significant difference （P 0.05）Conclusion Down-regulation of 15-LOX-1 can inhibit inflammatory cytokines， regulate PAECs proliferation， and improve endothelial injury induced by LPS. 　　[Key words] Pulmonary arterial endothelial cells； 15-lipoxygenase-1； Lipopolysaccheride； Inflammatory cytokines 　　肺炎是临床上常见病、多发病，如不能及时治疗，可导致呼吸衰竭、肺心病等，甚至死亡[1-2]。细菌脂多糖（Lipopolysaccheride，LPS）是细菌内毒素的主要成分，可参与诱导细胞损伤，加重炎症[3-4]。研究表明15-脂氧酶-1（15-LOX-1）可参与调控炎性反应[5-6]。本研究通过体外实验探讨下调15-LOX-1对LPS作用下肺动脉内皮细胞（PAECs）和炎性因