MHC抗原加工递呈HLA―DO基因多态性与HCV感染易感性分子流行病学研究.docVIP

MHC抗原加工递呈HLA―DO基因多态性与HCV感染易感性分子流行病学研究 　　【摘要】 目的 探?主要组织相容性复合体（MHC）抗原加工递呈相关基因HLA-DOA和HLA-DOB多态性与丙型肝炎病毒（HCV）易感性的关系。方法 选取994例静脉注射吸毒人群， 均进行检测血清中抗-HCV， 将抗-HCV阴性者493例作为抗-HCV阴性组， 抗-HCV阳性者501例作为抗-HCV阳性组。分析HLA-DOA和HLA-DOB基因SNPs与HCV感染易感性的关系以及环境-基因的交互作用。结果 研究对象中年龄最小12岁， 最大64岁， 抗-HCV阳性组平均年龄（36.0±7.5）岁， 抗-HCV阴性组平均年龄（34.0±7.9）岁；抗-HCV阳性组年龄略高于抗-HCV阴性组， 差异有统计学意义（P0.05）；非深圳户籍人口抗-HCV阳性率（51.8%）略高于深圳户籍（40.0%）， 差异有统计学意义（P0.05）。两组在文化程度上均以初中及以下为主， 但在构成上比较差异有统计学意义（P0.05）， 抗-HCV阴性组的高中及以上学历构成（24.3%）高于抗-HCV阳性组（17.0%）。抗-HCV阳性组谷丙转氨酶和谷草转氨酶的水平显著高于抗-HCV阴性组（P0.01）。在吸毒人群中， HLA-DOA rs3128935 T→C和HLA-DOB rs2071469 A→G突变的改变均能显著降低个体感染HCV的风险， 在调整了年龄、性别、户籍和文化程度以后， 携带突变等位基因纯合子的个体发生HCV感染的可能性降低一半， OR值分别为0.32和0.50， P0.01。结论 HLA-DOA rs3128935和HLA-DOB rs2071469突变的改变均能显著降低吸毒人群感染HCV的风险。 　　【关键词】 丙型肝炎病毒；遗传多态性；抗原加工递呈；感染结局；中国人群 　　DOI：10.14163/j.cnki.11-5547/r.2016.32.001 　　【Abstract】 Objective To investigate relationship between major histocompatibility complex （MHC） antigen processing machinery related HLA-DOA and HLA-DOB polymorphism and hepatitis C virus （HCV） infection susceptibility. Methods A total of 994 intravenous drug abuse people all received serum anti-HCV detection. 493 cases with negative anti-HCV were taken as negative anti-HCV group， and 501 cases with positive anti-HCV cases as positive anti-HCV group. Analysis was made on relationship between SNPs of HLA-DOA， HLA-DOB and HCV infection susceptibility， and reciprocal action of environment-gene. Results The youngest case aged 12 years old and the eldest case aged 64 years old， with mean age as （36.0±7.5） years old in positive anti-HCV group and （34.0±7.9） years old in negative anti-HCV group. Age in the positive anti-HCV group was slightly higher than the negative anti-HCV group， and the difference had statistical significance （P0.05）. Migrant population without registered Shenzhen residence had higher positive anti-HCV rate （51.8%） than drug abuse population with registered Shenzhen residence （40.0%）， and the difference had statistical significance