Caveolin1促进糖尿病大鼠缺血下肢血管形成作用及机制Caveolin1促进糖尿病大鼠缺血下肢血管形成作用及机制.docVIP

Caveolin1促进糖尿病大鼠缺血下肢血管形成作用及机制Caveolin1促进糖尿病大鼠缺血下肢血管形成作用及机制 　　[摘要] 目的 探讨Caveolin-1对下肢缺血糖尿病大鼠缺血部位血管形成的作用及与AKT信号通路的关系。 方法 健康、雄性SD大鼠，在腹腔注射链脲菌素构建的1型糖尿病大鼠模型上离断左侧股动脉及其分支，建立急性下肢缺血动物模型，14 d后用Western blot检测Caveolin-1、AKT的表达水平；28 d后进行CD34免疫组织化学染色和HE染色，评价血管密度。 结果 缺血组和糖尿病组与假手术组相比，Caveolin-1蛋白的表达水平明显增加（P0.05）；且缺血组Caveolin-1蛋白含量显著高于糖尿病组（P0.05）。缺血组、缺血转染组、缺血空转染组的Caveolin-1蛋白、AKT水平均明显高于假手术组（P0.05）；缺血转染组与缺血组相比，Caveolin-1蛋白、AKT的表达水平明显增多（P0.05）。与假手术组相比，缺血组、缺血转染组和缺血空转染组的微血管均有一定程度增加，其中缺血转染组的微血管密度增加尤为显著（P0.05）。 结论 Caveolin-1过表达后对糖尿病大鼠下肢缺血部位的血管形成有明显的促进作用，可能与激活AKT有关。 　　[关键词] Caveolin-1；糖尿病；下肢缺血 　　[中图分类号] R587.1[文献标识码] A[文章编号] 1674-4721（2014）06（c）-0004-05 　　 　　The effects and mechanism of Caveolin-1 on limb ischemia region of diabetic rat 　　XIONG Guo-zuo1 LIU Hui2 LI Tian-ping3 YAN Ya-ping1 SHEN Xin1 DAI Xian-peng1 DENG Li-ming1 BI Guo-shan1 HU Bing-bing1 　　1.Department of Vascular Surgery,the Second Affiliated Hospital.University of South China,Hengyang 421001,China;2.Central Hospital of Yiyang City in Hunan Province,Yiyang 413000,China;3.The Institute ofPharmacy Pharmacology,University of South China,Hengyang 421001,China 　　[Abstract] Objective To investigate the function of Caveolin-1 on angiogenesis in lower limb ischemia region of diabetic rat and the relationship with AKT signal pathway. Methods Intraperitoneal injection of healthy,male,SD rats with streptozocin to construct type 1 diabetic rat model,then broken the left femoral artery to set up the acute lower limb ischemia animal model.After 14 days with Western blot detection Caveolin-1 and AKT expression.CD34 immunohistochemical staining and HE staining was applied to detect the vascular density in 28 days. Results The level of Caveolin-1 in diabetes group and ischemia group was obviously higher than that in sham group respectively,the level of Caveolin-1 in ischemia group was higher than that in diabetes group,with statistical difference(P0.05).The level of Caveolin-1 and AKT in ischemia group,ischemic transfection group,