《烟草及烟草制品游离氨基酸测定离子-积分脉冲安培法》.docVIP

PAGE PAGE 23 《烟草及烟草制品 游离氨基酸的测定 离子色谱-积分脉冲安培法》 标准项目试验报告 山东中烟工业有限责任公司 2012-03 目 录 1. 立项背景························································4 标准适用范围····················································9 原理····························································9 实验方法························································9 仪器设备·····················································9 4.2试剂和材料···················································9 4.3样品前处理··················································10 4.4色谱条件····················································10 4.5标准曲线配制及制作··········································11 4.5.1标准溶液配制··········································· 4.5.2 标准曲线制作··········································· 结果与讨论·····················································13 5.1前处理······················································13 5.1.1 萃取方式的选择········································· 5.1.2 称样量的选择···········································13 5.1.3 萃取时间的优化·········································13 5.2仪器条件的选择··············································13 5.2.1固相萃取小柱选择······································· 5.2.2色谱柱温度的优化······································ 5.2.3进样量的选择··········································15 5.3分析参数····················································15 5.3.1样品测定结果··········································· 5.3.2 检测限················································· 5.3.3方法的重复性试验······································· 5.3.4 样品回收率·········································· 5.3.5与氨基酸分析法比对····································· 5.3.6比对实验··············································· 6 方法普适性······················································25 参考文献···························································26 1. 立项背景 氨基酸是烟草中主要的成分之一，它们与烟草的品质密切相关。游离氨基酸是烟叶中的重要化学成分，也是烟草中的一类重要致香前体物质，氨基酸的含量与烟草的品质有着密切的关系，其含量的高低直接影响到烟的味道和烟气的丰满度[1、2]，在烟叶的烘烤、陈化、调制及抽吸过程中均会与烟叶中的还原糖发生梅拉德反应而形成多种重要的致香成分，故烟叶中游离氨基酸种类的多少和含量的高低会直接影响烟叶的内在品