不同水分条件对三七种子后熟与萌发生理影响研究.docVIP

不同水分条件对三七种子后熟与萌发的生理影响研究 　　[摘要] 以三七种子为试验材料，研究了不同水分条件对三七种子后熟及萌发过程中种子含水量、活力和抗氧化酶等生理指标的影响。结果表明，与25%含水量沙埋层积处理相比，5%含水量沙埋层积处理下三七种子含水量稳定，种子活力比其高51%，抗氧化酶（SOD，POD，CAT）活性和丙二醛（MDA）含量变化较小，粗脂肪和可溶性总糖含量显著降低。随PEG 6000 处理浓度的升高，三七种子的萌发特性指标均显著降低；抗氧化酶活性先升高后降低，丙二醛含量，水溶性蛋白和总糖含量显著增加；萌发特性指标与渗透物质含量均呈极显著正相关（r0900，P0900，P001）。综上所述，三七种子因具有脱水不耐受特性，最佳沙埋含水量为5%，成熟后萌发过程最大PEG 6000耐受浓度为15%。 　　[关键词] 三七； 顽拗性种子； 后熟过程； 萌发特性； 抗氧化酶； 渗透物质 　　Effect of different water conditions on Panax notoginseng seeds afterripening and germination physiology 　　LIAO Peiran， CUI Xiuming*， YANG Ye*， QU Yuan， WANG Chengxiao， YANG Xiaoyan， XIONG Yin 　　（Key Laboratory of Panax notoginseng Resources Sustainable Development and Utilization 　　of State Administration of Traditional Chinese Medicine， Kunming Key Laboratory of Sustainable 　　Development and Utilization of FamousRegion Drug， Faculty of Life Science and Technology， 　　Kunming University of Science and Technology， Kunming 650500， China） 　　[Abstract] Effect of different water conditions on the physiological indexes （egseed water content， vigor， antioxidase activities）of Panax notoginseng seeds were studied under process of afterripening and germinationThe results showed show that compared with 25% treatment， under the treatment of 5%， Pnotoginseng seeds possessed stable seed water content， the seed vigor was exceed by 51%，variation of antioxidant enzyme （SOD， POD， CAT） activity and malondialdehyde （MDA） content were small， crude fat and total sugar content decreased significantlyWith the increase of PEG 6000 concentration， the germination characteristic indexes obviously decreased， antioxidase activities increased firstly and decreased afterwards， content of MDA， soluble protein and total sugar increased obviouslyThere were significant positive correlation between germination characteristic indexes and osmotic substance content（r0900， P0900， P001）In conclusion， because the characteristic of dehydration intolerance of Pnotoginseng seeds， 5% water content of sand bu