二氢杨梅素对肝纤维化大鼠肝功能及肝纤维化指标影响.docVIP

二氢杨梅素对肝纤维化大鼠肝功能及肝纤维化指标的影响 　　【摘要】目的：观察二氢杨梅素对四氯化碳（CCL4）所致肝纤维化大鼠血清肝功能及肝纤维化指标的影响。方法：84只雄性SD大鼠随机分为正常对照组、模型组、复方鳖甲阳性对照组和二氢杨梅素低、中、高剂量组。采用40%CCL4皮下注射制备大鼠肝纤维化模型，造模的同时分别用3种不同浓度的二氢杨梅素进行干预，持续13周。测定各组大鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）及透明质酸（HA）、层粘连蛋白（LN）、Ⅲ型前胶原（PC-Ⅲ）、Ⅳ型胶原（CⅣ）的含量。结果：与正常组比较，模型组大鼠血清中ALT、AST及HA、LN、PC-Ⅲ、CⅣ的含量均显著升高（P0.01）；与模型组比较，复方鳖甲组及二氢杨梅素低、中、高剂量组大鼠血清中ALT、AST及HA、LN、PC-Ⅲ、CⅣ的含量均显著降低（P0.05）。结论：二氢杨梅素对大鼠实验性肝损伤具有一定的预防作用和抗肝纤维化作用。 　　【关键词】二氢杨梅素；肝纤维化；谷丙转氨酶；谷草转氨酶；透明质酸；层粘连蛋白；Ⅲ型前胶原；Ⅳ型胶原 　　【中图分类号】R657.3+1【文献标识码】A【文章编号】1007-8517(2009)08-0006-02 　　 　　Effects of Dihydromyricetin on the content of ALT, AST, HA, LN, PC-Ⅲ, CⅣ in serums 　　on hepatic fibrosis in rats 　　KUANG Manyuan, JIA Lei, LUO Mingying 　　（Department of Anatomy Xiangnan Medical College, Chenzhou, 423000, China） 　　【Abstract】 Objective: To observe the effects of dihydromyricetin on the content of ALT, AST, HA, LN, PC-Ⅲ, CⅣ in serums on hepatic fibrosis in rats induced by carbon tetrachloride(CCL4). Methods: Eighty four male SD rats were randomly divided into six groups: normal group, model group, Fufangbiejiaruanganpian group positive, lower, middle and high dosage of dihydromyricetin treatment groups. The hepatic fibrosis rat model was developed by subcutaneous injection of 40%CCL4 for 13 weeks. At the same time of modeling, rats were treated with dihydromyricetin at 25mg/100g, 50mg/100g or 100mg/100g.Then the content of ALT, AST, HA, LN, PC-Ⅲ, CⅣ were detected in liver serums. Results: Compared with control group, the content of ALT, AST, HA, LN, PC-Ⅲ, CⅣ in serums of rats of model group all raised markedly(P0.01). Compared with model group, the content of ALT, AST, HA, LN, PC-Ⅲ, CⅣ in serums of rats of Fufangbiejiaruanganpian group positive and lower, middle and high dosage of Dihydromyricetin treatment groups reduced markedly(P0.05). Conclusion: Dihydromyricetin can protect hepatocytes from injury and prevent the progression of hepatic fibrosis in rats. 　　【Keywords】Dihydromyricetin; Hepatic fibrosis; alanine aminotran