第十二章 细胞的衰老和死亡.pptVIP

一、细胞凋亡与细胞坏死的区别 流式细胞仪分析（FACS） （三）细胞内外因素主要通过两种信号传导途径诱导细胞凋亡 1.死亡受体介导的细胞凋亡信号通路 2.线粒体介导的细胞凋亡信号通路 * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * While the annexin-V binding assay is fairly robust when used in suspension growing cells, its use can be a little tricky when assessing apoptosis in adherent cells. The most common methods of cell detachment use trypsinisation and/or EDTA, both of which can seriously affect the assay. Trypsinisation can affect both membrane integrity and PS localisation and EDTA will sequester Ca2+, which is essential for the binding of annexin-V.