凝血酶敏感蛋白1对胆囊癌细胞生长增殖作用.docVIP

凝血酶敏感蛋白1对胆囊癌细胞生长增殖作用 　　【摘要】 目的 探讨研究凝血酶敏感蛋白-1(TSP-1)对胆囊癌细胞株GBC-SD生长增殖的作用及其作用途径。方法 根据处理因素将胆囊癌细胞分为：空白对照组，TSP-1组，CD36单抗阻断组、CD47单抗阻断组。用四唑盐比色法(MTT)和流式细胞术检测TSP-1及受体CD36、CD47对胆囊癌细胞生长、细胞周期的影响。结果 TSP-1组、CD36阻断组、CD47阻断组细胞生长明显低于对照组(抑制率分别为：23．63%，9．57%，18．13%)。CD36阻断组的抑制率小于CD47阻断组。TSP-1组G0/G1期细胞数增加，S期和G2/M期细胞数减少，与对照组和CD36阻断组相比有显著性差异。增殖指数分别为：对照组(39．55±2．12)、TSP-1组(26．21±0．61)、CD36阻断组(36．43±1．28)、CD47阻断组(30．73±0．38)；各组之间两两比较均有差异。结论 TSP-1可对抑制胆囊癌细胞的生长，通过与受体CD36的结合阻滞胆囊癌细胞停止于G0/G1期可能是主要的作用途径。? 　　【关键词】胆囊癌;凝血酶敏感蛋白-1；细胞周期，生长，增殖 　　 　　The influence of TSP-1 on growth of gallbladder carcinoma cell 　　 　　XUE Jian-feng,FAN Zheng-jun,ZHAO Shou-kui,et al.Department of Surgery,First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China 　　? 　　【Abstract】 Objective To explored the role and the mechanism of TSP-1 on the growth and cell cycle of of gallbladder carcinoma cellline GBC-SD.Methods The cell line was divided into 4 groups：control group,TSP-1 group,CD36-blocked group,CD47-blocked group.To detect the role of TSP-1,CD36,CD47 on cell growth by MTT test,on cell cycle by Flow cytometry.Results The growth of GBC-SD in other group were significantly slower than in control group,inhibitory rate was respectively 23．63%，9．57%，18．13%.Inhibitory effect of growth in CD36-blocked group was lower than in CD47-blocked group.Flow cytometry indicated that the cell number in G0/G1 phase in TSP-1 group increased than that in control group and CD36-blocked group,the cell number in S and G2/M phase in TSP-1 group reduced than in control group.The proliferation index was respectively：（39．55±2．12）(control group)、（26．21±0．61）(TSP-1 group)、（36．43±1．28）(CD36-blocked group)、（30．73±0．38）(CD47-blocked group)，the difference among groups were significantly.Conclusion TSP-1 can inhibit the growth of HCCLM3 cell through combining with the receptor CD36.? 　　【Key words】Gallbladder carcinoma；Thrombospondin-1,cell cycle；Growth;Proliferation 　　 　　胆囊癌的发病率近年来有增加的趋势，发现时已多属中晚期，手术切除率低，生长快，恶性程度高[1]，胆囊癌的生长增殖是在体内各种生