化瘀祛浊复方对糖尿病大鼠大血管病变炎性因子影响.docVIP

化瘀祛浊复方对糖尿病大鼠大血管病变炎性因子影响 　　[摘要] 目的 观察化瘀祛浊复方对糖尿病大鼠大血管病变炎性因子的影响。 方法 应用链尿佐菌素复制糖尿病大鼠大血管病的病理模型，大鼠的血糖值11.2 mmol/L为造模成功列入观察对象，然后共分为6组，实验期间，每组予以不同的饲养。实验期间大鼠尾尖采血，实验8周后腹主动脉采血，待放射免疫法检测血清中肿瘤坏死因子（tumor necrosis factor-a，TNF-a），且观察其变化。结果 模型组大鼠与正常组比较TNF-a明显升高，差异有统计学意义（P0.01）；AS组、治疗组比较TNF-a升高其次（P0.05），阳性药组与预防组差异无统计学意义。应用化瘀祛浊中药复方影响炎症水平。结论 应用化瘀祛浊复方可以抑制肿瘤坏死因子的表达，使糖尿病大血管病的炎性因子处于炎症状态，从而能够延缓糖尿病合并症的进展。 　　[关键词] 化瘀祛浊；糖尿病大血管病；大鼠；肿瘤坏死因子 　　[中图分类号] R587.2 [文献标识码] A [文章编号] 1672-4062（2016）12（b）-0005-02 　　[Abstract] Objective To observe the effect of the compound prescription of removing blood stasis and removing turbid on the inflammatory factors of the large blood vessel pathological changes in diabetic rats. Methods The pathological model by streptozotocin to copy large vessel disease in diabetic rats， rats blood glucose greater than 11.2 mmol/L for the successful modeling included in the observation objects， and were divided into 6 groups， each group during the experiment， give the different feeding. During the experiment， blood samples were collected at the end of the experiment， blood samples were collected from abdominal aorta 8 weeks later， and necrosis factor-A tumor（TNF-a） was detected by radioimmunoassay， and the changes were observed. Results Compared with the normal group， the TNF-a in model group was significantly increased， and the difference was significant（P0.01）； AS group， treatment group compared to TNF-a increased second（P 0.05）， positive drug group and prevention group were not significantly different. Effect of TCM compound prescription of removing blood stasis and removing turbid to influence the level of inflammation. Conclusion The application of removing blood stasis and removing turbid compound can inhibit the expression of tumor necrosis factor， so that the inflammatory factor of diabetic vascular disease in the inflammatory state， so as to delay the progress of diabetic complications. 　　[Key words] Removing stasis and removing turbidity； Diabetic vascular disease；