Forensic DNA TypingorDid you kill (rape…) that personHow.pptVIP

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2018-08-28 发布于江苏
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Forensic DNA TypingorDid you kill (rape…) that personHow.ppt

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Forensic DNA TypingorDid you kill (rape…) that personHow

Forensic DNA TypingorDid you kill (rape…) that person?How DNA can “definitively” say.Adapted from:National Institutes of Science Technology /div831/strbase/intro.htm Brief History of Forensic DNA Typing 1980 - Ray White describes first polymorphic RFLP marker (Restriction Fragment Length Polymorphism [alleles]). Different RFLP for different people 1985 - Alec Jeffreys discovers multilocus VNTR (variable number of tandem repeats) probes. 1985 - first paper on PCR 1988 - FBI starts DNA casework 1991 - first STR paper (renaming of VNTR– could be larger repeats, STR 4-6 bp’s. now using mostly 4 bases ) 1995 - FSS (Forensic Science Service-UK) starts UK DNA database 1998 - FBI launches CODIS (Combined DNA Information Service) Now FBI use 13 loci: PCR identifies it: in the quadrillions – except for identical. Except for police mistakes, it’s done deal. RFLP’s: Sickle Cell hemoglobinCase 1: Screening for the sickle-cell gene Brief History of Forensic DNA Typing 1980 - Ray White describes first polymorphic RFLP (Restriction Fragment Length Polymorphism) marker—detect to transferring to membrane. Probe w southern blot (radiological). Diff. RFLP for dif. People. Single rflp 1985 - Alec Jeffreys discovers multilocus VNTR (variable number of tandem repeats) probes (stat. very impressive identical 4-6 bp that are spec. 7 and 9 repeat, one from mom and dad, on chrom. 1- nowadays use pcr- but flanking sequence that is unique to chromo1)). Jeffreys almost ident. Typing. Now use PCR. 1985 - first paper on PCR (Kerry Mullis) 1988 - FBI starts DNA casework 1991 - first STR paper ( renaming of VNTR– could be larger repeats, STR 4-6 bp’s. now using mostly 4 bases ) 1995 - FSS (Forensic Science Service-UK) starts UK DNA database 1998 - FBI launches CODIS (Combined DNA Information Service) database. Now FBI use 13 loci: PCR identifies it: in the quadrilians – except for identical. DNA Use in Forensic Cases Most are rape cases (2 out of 3) Looking for match between eviden