参芪抑瘤方药物血清对胃癌MGC―803细胞增殖影响.docVIP

参芪抑瘤方药物血清对胃癌MGC―803细胞增殖影响 　　摘要：目的 观察参芪抑瘤方药物血清对胃癌MGC-803细胞增殖的影响，探讨其相关机制。方法 以参芪抑瘤方不同浓度药物血清干预胃癌MGC-803细胞后，MTT法检测细胞增殖，流式细胞仪检测细胞周期，qRT-PCR检测CDKN1B、CDKN1C基因表达，Western blot检测p27、p57蛋白表达。结果 3%、5%、10%药物血清作用于MGC-803细胞24、48、72 h，细胞增殖水平显著降低（P0.01），且与时间和剂量呈依赖关系；3%、5%、10%药物血清作用于MGC-803细胞24 h后，G0/G1期细胞增多，S期细胞减少；qRT-PCR检测结果显示，与对照组和空白血清组比较，药物血清各剂量组MGC-803细胞CDKN1B、CDKN1C mRNA表达显著上调（P0.01）；Western blot检测结果显示，与对照组比较，药物血清各剂量组MGC-803细胞p27、p57蛋白表达显著上调（P0.01）。?Y论 参芪抑瘤方药物血清可抑制胃癌MGC-803细胞的增殖，其机制可能是通过调节CDKN1B、CDKN1C mRNA和蛋白表达，进而干预细胞周期。 　　关键词：参芪抑瘤方；药物血清；胃癌细胞；细胞增殖；细胞周期 　　DOI：10.3969/j.issn.1005-5304.2017.03.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）03-0053-04 　　Effects of Medicinal Serum of Shenqi Yiliu Formula on Proliferation of Gastric Cancer MGC-803 Cells MA Chun-lin1， WU Hong-yan1，2，3， LI Hai-long1，2， CHEN Jie1， ZHANG Xuan1， LI Hong-liang1 （1. Gansu University of Chinese Medicine， Lanzhou 730000， China； 2. State Key Laboratory of Traditional Chinese Medicine Excavation Innovation and Transformation of Gansu Province， Lanzhou 730000， China； 3. Laboratory for TCM New Products Development Engineering of Gansu Province， Lanzhou 730000， China） 　　Abstract： Objective To investigate the inhibitory effects of medicinal serum of Shenqi Yiliu Formula on cell proliferation of gastric cancer MGC-803 cells； To discuss relevant mechanism. Methods After treated with different concentrations of medicinal serum of Shenqi Yiliu Formula， gastric cancer MGC-803 cells were tested by the following methods： MTT was employed to test the proliferation of gastric cancer MGC-803 cells； Flow cytometry was used to detect cell cycle； qRT-PCR was used to detect the genetic expressions of CDKN1B and CDKN1C； Western blot was employed to test the protein expressions of p27 and p57. Results When 3%， 5% and 10% medicinal serum of Shenqi Yiliu Formula was treated to MGC-803 cells for 24 h， 48 h and 72 h， proliferation of cells decreased significantly （P 　　Key words： Shenqi Yiliu Formula； medicinal serum； gastric c