参芪醒脑方对痴呆模型小鼠学习记忆能力及脑组织胆碱乙酰转移酶乙酰胆碱酯酶含量影响.docVIP

参芪醒脑方对痴呆模型小鼠学习记忆能力及脑组织胆碱乙酰转移酶乙酰胆碱酯酶含量影响 　　摘要：目的 观察参芪醒脑方对氢溴酸东莨菪碱致痴呆模型小鼠学习记忆能力及脑组织胆碱乙酰转移酶（ChAT）、乙酰胆碱酯酶（AChE）含量的影响，探讨其防治老年痴呆的可能作用机制。方法 将ICR小鼠110只随机分为对照组、对照+参芪醒脑方高剂量组、模型组、多奈哌齐组和参芪醒脑方低、中、高剂量组。对照组和模型组小鼠给予蒸馏水灌胃，其余各组小鼠给予相应药物灌胃，每日1次，连续14 d。第15日Morris水迷宫实验、物体识别实验评价小鼠学习和记忆能力，行为学测试开始前20 min氢溴酸东莨菪碱腹腔注射诱发小鼠记忆障碍建立模型。Western blot检测小鼠皮层及海马组织ChAT、AChE蛋白表达。结果 Morris水迷宫实验结果显示，与对照组比较，模型组小鼠逃避潜伏期明显延长（P0.01）；与模型组比较，参芪醒脑方中、高剂量组小鼠逃避潜伏期明显缩短（P0.05）。物体识别实验结果显示，与对照组比较，模型组小鼠对新事物探究能力明显下降、分辨指数明显降低（P0.001）；与模型组比较，参芪醒脑方各剂量组小鼠分辨指数明显升高（P0.05，P0.01，P0.001）。Western blot结果显示，与对照组比较，模型组小鼠脑皮层及海马组织AChE蛋白表达明显升高（P0.01）；与模型组比较，参芪醒脑方低、中剂量组小鼠脑皮层AChE蛋白表达明显降低（P0.01），参芪醒脑方各剂量组小鼠脑海?R组织AChE蛋白表达明显降低（P0.001），参芪醒脑方各剂量组小鼠脑皮层及海马组织ChAT蛋白表达均未见明显差异。结论 参芪醒脑方能明显改善氢溴酸东莨菪碱致痴呆模型小鼠的学习记忆能力，可能与降低脑内AChE蛋白表达有关。 　　关键词：参芪醒脑方；阿尔茨海默病；东莨菪碱；胆碱乙酰转移酶；乙酰胆碱酯酶；小鼠 　　DOI：10.3969/j.issn.1005-5304.2018.04.012 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）04-0056-06 　　Abstract： Objective To observe the effects of Shenqi Xingnao Prescription on learning and memory ability， contents of choline acetyltransferase （ChAT） and acetylcholine esterase （AChE） in brain tissue in mice models with scopolamine-induced Alzheimer disease （AD）； To investigate its mechanism for prevention and treatment for AD. Methods Totally 110 ICR mice were randomly divided into control group， control+Shenqi Xingnao Prescription high-dose group， model group， donepezil group， model+Shenqi Xingnao Prescription high-， medium-， and low-dose groups. The control and model group were given distilled water for gavage， and the other groups were given the corresponding medicine for gavage， once a day， for 14 days. On the 15th day， Morris water maze test and object recognition test were used to evaluate the learning and memory ability of each group. The model mice of memory impairment induced by intraperitoneal injection of scopolamine was established 20 minutes before the behavioral test. The expressions of ChAT and AChE in cortex and hippocampus were detected by Western blot. Results The