参萸补血方对再生障碍性贫血模型小鼠Th17细胞及其相关因子IL―6IL―17及IL―23表达水平影响.docVIP

参萸补血方对再生障碍性贫血模型小鼠Th17细胞及其相关因子IL―6IL―17及IL―23表达水平影响 　　[摘要] 目的 ?^察参萸补血方对再生障碍性贫血（AA）模型小鼠Th17细胞及其相关因子白细胞介素6（IL-6）、IL-17及IL-23表达水平的影响，探讨其治疗AA的效果。 方法 选取清洁级BALB/c小鼠80只，按随机数字表法分为正常对照组、模型组、参萸补血方组、强的松（阳性对照）组。以60CO-γ射线照射加腹腔注射环磷酰胺（CY）加氯霉素（CH）复合方法建立AA小鼠模型。造模成功后给药干预，连续4周后观察各组小鼠外周血象改变及各组小鼠Th17细胞及其相关因子IL-6、IL-17及IL-23表达水平的变化，光镜下观察骨髓增生程度。 结果 与正常对照组比较，模型组小鼠外周血象和骨髓增生明显降低，Th17细胞及其相关因子IL-6、IL-23和IL-17表达均显著升高，差异有统计学意义（P 0.05）。与模型组比较，参萸补血方组小鼠外周血像明显升高，Th17细胞及其相关因子IL-6、IL-23和IL-17表达水平下降，骨髓增生明显改善，差异有统计学意义（P 0.05）。 结论 参萸补血方可改善AA模型小鼠的骨髓造血功能，可能与其抑制T细胞免疫功能相关的IL-6、IL-17及IL-23表达水平有关。 　　[关键词] 再生障碍性贫血；参萸补血方；Th17细胞；白介素17；白介素6 　　[中图分类号] R556.53 [文献标识码] A [文章编号] 1673-7210（2018）04（c）-0013-04 　　Effects of Shenyu Buxue Decotion on the Th17 cells and expression of its related IL-6， IL-17 and IL-23 expression in mice with aplastic anemia 　　ZENG Qing1 LU Haisong1 CHENG Weimin1 HE Ai1 DENG Dejun2 LAI Chunfeng3 　　1.Department of Hematology， the First Affiliated Hospital of Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 530023， China； 2.Graduate School， Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 500001， China； 3.Department of Oncology， Yullin Traditional Chinese Medicine Hospital， Guangxi Zhuang Autonomous Region， Yulin 537000， China 　　[Abstract] Objective To observe the effect of Shenyu Buxue Decotion on Th17 cells and the expression of IL-6， IL-17 and IL-23 in aplastic anemia （AA） model mice， and to explore its curative effect for AA. Methods Eighty clean-class BALB/c mice were divided into control group， model group， Shenyu Buxue Decotion group and Prednisone （positive control） group by random number table. The AA models were established by irradiated with 60CO-γ ray together with intraperitoneal injection of cyclophosphamide （CY） and chloramphenicol （CH）. After AA models were successfully established， mice were given to medicines for 4 weeks. The changes of mice peripheral hemogram， Th17 cells and the expression levels of IL