基于HPLC指纹图谱对救必应与其伪品鉴别研究.docVIP

基于HPLC指纹图谱对救必应与其伪品鉴别研究 　　摘要：目的 建立救必应与其伪品米碎木的HPLC指纹图谱，进行真伪鉴别。方法 收集各地名为“救必应”的药材，采用HPLC指纹图谱方法对救必应及其伪品米碎木进行检测，采用《中药色谱指纹图谱相似度评价系统2004A版》对指纹图谱进行评价。结果 建立了救必应和米碎木的HPLC指纹图谱，方法学考察符合指纹图谱技术要求。救必应与其伪品米碎木指纹图谱轮廓特征明显不同，救必应有31个共有峰，米碎木有28个共有峰。主成分分析可将样品分为2组，聚类分析也可将样品分为2类。结论 采用HPLC指纹图谱及其数据分析，可以对救必应与其伪品进行鉴别，为救必应的质量评价提供有效方法。 　　关键词：救必应；米碎木；高效液相色谱法；指纹图谱；主成分分析；质量控制 　　DOI：10.3969/j.issn.1005-5304.2017.08.020 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）08-0089-04 　　Identification of Ilicis Rotundae Cortex and Its Adulterant Based on HPLC Fingerprint MA Yu-cui1， WANG Chun1， WANG Wei2， WU Cui1， DU Ning2， SUN Wen1， LI Hui1， CHAO Zhi-mao1 （1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China； 2. Beijing Center for Physical and Chemical Analysis， Beijing 100094， China） 　　Abstract： Objective To establish HPLC fingerprints for the identification of Ilicis Rotundae Cortex （Jiubiying）， dried bark of Ilex rotunda Thunb.， and its adulterant， dried bark of Ilex godajam （Colebr.） Wall.. Methods Some samples named “Jiubiying” were collected from some medicine markets and drugstores. The HPLC fingerprints were obtained to detect Jiubiying and Ilex godajam. The software “Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine （2004A edition）” was used to evaluate fingerprints. Results The fingerprints of Jiubiying and Ilex godajam were established. Methodological study met the technical requirements of fingerprints. There were 31 and 28 common peaks in Jiubiying and Ilex godajam， respectively. Jiubiying and Ilex godajam could be classified into two clusters by principal component analysis and hierarchical clustering analysis. Conclusion Jiubiying and Ilex godajam can be identified by HPLC fingerprints. This method can provide an effective method for quality evaluation of Jiubiying. 　　Key words： Ilicis Rotundae Cortex （Jiubiying）； Ilex godajam （Colebr） Wall.； HPLC； fingerprint； principal component analysis； quality