膜分离技术MembraneSeparation-宜兰大学食品科学系-国立宜兰大学.PPTVIP

Pulsed Electric Fields processing Mechanisms of Microbial Inactivation Electroporation(電穿孔、電擊通透) (Vega-Mercado, 1996) ? Thank you 國立宜蘭大學 食品科學系 馮臨惠 Separation Processes Selection of Operations in Separation Processes Chemical, Physical, and Biochemical Concepts in Isolation and Purification of Proteins Selection of Operations in Separation Processes Defining Final Product Characterization of Starting Material Selecting of Separation Sequence Purification Process and Unit Operations Protein Recovery Protein Purification Protein separation techniques Protein Recovery Cell separation Cell disruption and debris separation (for intracellular proteins only) Concentration Cell disruption Different types of cells (e.g. microbial, animal and plant) produce proteins either intracellular or extracellular For recovering intracellular proteins, the cells have to be disrupted Disruption of microorganisms Release of intracellular proteins Release of intracellular enzymes Batch and continuous flow cell disruption Degree of disruption Reproducibility Practical application The ideal technology for cell Disruption Maximum release of the product of interest No mechanical or thermal denaturation of the product during disruption Minimal release of proteases which may degrade the product Minimal release of particulates or soluble contaminants that may influence downstream processing The desired attributes of mechanical cell disruption equipment Low capital and operating costs Could be Sterilized May be cleaned in place (CIP) May be validated for cGMP requirements Scalable (scale up) May be automated Process Design Considerations Disruption Kinetics Operating Pressure Energy Requirements Protein Denaturation Valve Design Cell Physiological Factors Scale up Enzyme Release Application Disruption and homogenization of cells Different techniques: shearing, grinding, sonication, French pressure cell disruption, depending of cell types Isolation of organelles; solubilizatio