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白藜芦醇对C6鼠胶质瘤细胞抑制生长和诱导凋亡的作用 王广秀 浦佩玉 康春生 王虎 董伦 李彦和 张志勇 [摘要] 目的 研究白藜芦醇（resveratrol，Res）在体外抑制C6鼠胶质瘤细胞增殖、诱导凋亡及其作用机制。方法 以不同浓度Res处理C6鼠胶质瘤细胞24，48，72，96h，用MTT法检测其对细胞生长增殖的抑制作用，用流式细胞术（FCM）检测其对细胞周期及凋亡的影响，用蛋白印迹（Western　blot）检测Res处理前后C6细胞EGFR、GFAP、Bcl-2及Caspase-3的表达。结果 Res抑制C6鼠胶质瘤细胞的增殖呈浓度和时间依赖性，细胞被阻滞于S期，明显诱导细胞凋亡，Res可抑制EGFR、Bcl-2蛋白表达，而上调GFAP、 Caspase-3 表达。结论 Res可以下调EGFR及其信号转导通路抑制胶质瘤细胞增殖、促进其凋亡，有潜在的临床应用价值。 [主题词] 白藜芦醇；C6鼠胶质瘤细胞；增殖；凋亡；表皮生长因子受体 Effects of resveratrol in inhibiting growth of rat C6 glioma and inducing its apoptosis WANG Guangxiu PU Peiyu KANG Chunsheng WANG Hu DONG Lun LI Yanhe ZHANG Zhiyong Department of neurosurgery，Tianjin Medical University General Hospital， Tianjin 300052，China Corresponding author：PU Peiyu，E-mail： HYPERLINK mailto:ppyu@public.tpt.tj.cn ppyu@public.tpt.tj.cn [Abstract] Objective To investigate the effect of resveratrol on proliferation and apoptosis of C6 rat glioma cells in vitro. Methods C6 rat glioma cells were treated with resveratrol at various concentrations. MTT assay was used to determine the inhibitory rate of cell growth at 24，48，72 and 96 hours and flow cytometry was used to detect cell cycle and apoptosis status before and after the resveratrol treatment ，Western blot was used to examine the expression of EGFR，GFAP，Bcl-2 and Caspase-3. Results Resveratrol inhibited the growth of C6 rat glioma cells in a dose-and time-depentent manner and induced cell apoptosis。Cell cycle was blocked in S phase。Western 作者单位: 300052 天津医科大学总医院神经外科，天津市神经病学研究所，神经肿瘤实验室blot showed that resveratrol downregulated the expression of EGFR and Bcl-2 while upregulated the GFAP and Caspase-3 expression . Conclusion Resveratrol could inhibit glioma cell proliferation and induce the cell differentiation and apoptosis possibly through downregulation of EGFR signal pathway. 作者单位: 300052 天津医科大学总医院神经外科，天津市神经病学研究所，神经肿瘤实验室 Keywords] Resveratrol；glioma；apoptosis；EGFR 白藜芦醇（Resveratrol）是一种广泛存在于植物中的植物补体，Res不但可以作为一种抗氧化剂，有防治心血管疾病的功效。而且具有抗炎和抗癌的作用[1,2]，它可以在肿瘤的