基于气味客观化黄连及其炮制品鉴别研究.docVIP

基于气味客观化黄连及其炮制品鉴别研究 　　[摘要]气味是鉴别黄连炮制品的重要评价指标，其不同炮制品具有各自独特的气味。该研究以气味客观化为切入点，采用电子鼻技术，建立适合黄连的电子鼻检测方法，分别对生黄连、酒黄连、姜黄连及萸黄连的气味进行检测，依据获得的客观化气味信息，结合化学计量学方法对黄连及其不同炮制品进行区分判别。结果表明，黄连及其不同炮制品在气味特征上存在显著差异，统计质量控制分析（SQC）与软独立建模分析（SIMCA）模型能够实现黄连炮制品与生品的区分，主成分分析（PCA）分析可明显区分黄连及其不同炮制品;另外，对黄连及不同炮制品的区分识别，判别因子分析（DFA）模型正确判别率为100%，线性判别分析（LDA）模型的初始判别率以及交叉验证识别率分别为100%，94.4%。该研究采用电子鼻技术实现了黄连及其炮制品的气味特征差异表征，与化学计量学方法结合实现了黄连及其不同炮制品的鉴别区分;该研究为中药气味客观化鉴别研究提供了思路与方法，有利于传统气味鉴别经验的传承与发展。 　　[关键词]黄连;气味;客观化;炮制品;电子鼻; 化学计量学;鉴别 　　Discrimination of Coptidis Rhizoma and its processed products by odor objectify 　　XU Min1， YANG Shi-long1， ZHANG Chao1， WAN Jun2， WU Na1， LI Xin-yi1， HUANG Qin-wan1， ZHOU Xia2， WU Chun-jie1* 　　（1.Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China; 　　2. Southwest Jiaotong University， Chengdu 610031， China） 　　[Abstract]Different processed products of Coptidis Rhizoma have its unique odor， which is an important assessment index for processed products identification of Coptidis Rhizoma. Objectify odor as an entry point in this study， an electronic nose technology was used， and a suitable method for Coptidis Rhizoma measurement was built firstly. Then different processed products of Coptidis Rhizoma were detected by the method built. Finally， different processed products were identified by combining with chemometrics based on the objective odor information obtained. Electronic nose detection indicated that a significant difference in odor between different processed products was performed. Coptidis Rhizoma processed or not can be distinguished based on statistical quality control （SQC） and soft independent modeling of class analogy （SIMCA）. Principle component analysis （PCA） model showed that Coptidis Rhizoma and its various processed products discriminated obviously. In addition， in order to identify the processed products of Coptidis Rhizoma， a correct recognition rate of 100% was acquired by discriminant factor analysis （DFA）， and the initial identification rate a