水稻基因osasie1 抗逆功能解析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2011, 37(10): 1771−1778 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.01771 水稻基因OsASIE1 抗逆功能分析 吴慧敏 黄立钰 潘雅娇 靳 鹏 傅彬英* / , 100081 : , AP2/EREBP AP2/EREBP EREBP OsASIE1 (abiotic stress induced EREBP gene), OsASIE1 (electrophoresis mobility shift assay, EMSA), AP2 DRE (dehydration-responsive element)GCC box (ethylene response ele- ment), OsASIE1 DRE GCC box , : ; AP2/EREBP ; EMSA; ; Function Analysis of Gene OsASIE1 Responding to Abiotic Stresses in Rice * WU Hui-Min, HUANG Li-Yu, PAN Ya-Jiao, JIN Peng, and FU Bin-Ying Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China Abstract: AP2/EREBP transcription factors play an important role in plant development, hormone response, biotic and abiotic stress responses. We identified that OsASIE1, a member of EREBP subfamily of AP2/EREBP transcription factor family in rice, was involved in abiotic stress response. The expression of OsASIE1 was induced by drought and salt stresses, and over-expression of OsASIE1 in the transgenic rice plant could improve its tolerance to salt stress. Further electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of OsASIE1 protein could bind both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) in vitro. All these results implicated that OsASIE1 might be involved in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box bi