壳聚糖絮凝法精制补肾养血颗粒水提液工艺研究.docVIP

壳聚糖絮凝法精制补肾养血颗粒水提液工艺研究 　　摘要：目的 优选壳聚糖精制补肾养血颗粒水提液的工艺。方法 以淫羊藿苷为指标，应用高效液相色谱法测定其含量，采用正交设计法考察加水量、提取时间、提取次数对水提工艺的影响；以淫羊藿苷为指标，结合总多糖含量，选择药液浓度、絮凝温度、澄清剂加入量3个因素，优选壳聚糖的絮凝澄清条件。结果 最佳水提工艺条件为A2B1C3，即加入10倍量水，提取3次，每次1 h；最佳絮凝工艺为A1B2C3，即药液浓度为0.4 g/mL，絮凝温度为40 ℃，壳聚糖加入量为0.1%。结论 本研究优选的精制工艺稳定可行。 　　关键词：补肾养血颗粒；淫羊藿苷；壳聚糖；精制工艺；正交试验 　　DOI：10.3969/j.issn.1005-5304.2016.04.026 　　中图分类号：R283.5 文献标识码：A 文章编号：1005-5304（2016）04-0098-04 　　Study on Refining Water Extract of Bushen Yangxue Granules by Chitosan Flocculation DU Yan-jiao1，2， KANG Chen2， DU Mao-bo2， SHEN Shuo2， LI Man-ling2， LIANG Li-na3， LIU Shu-zhi2 （1. Jiangxi University of Traditional Chinese Medicine， Nanchang 330004， China； 2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China； 3. Eye Hospital， China Academy of Chinese Medical Sciences， Beijing 100040， China） 　　Abstract： Objective To optimize refinement of water extract from Bushen Yangxue Granules by chitosan flocculation. Methods According to the content of icariin detected by HPLC， the waters amount， extraction time and extraction times were evaluated by orthogonal design. The effects of the solution concentration， clarifying temperature and the amount of clarifying agent on the flocculation clarification processes were optimized with the content of icariin and polysaccharides. Results The optimum water extraction processes A2B1C3 were follows： 10 times amount of water， three times extraction and 1 h for each extraction process. The optimized flocculation clarification processes A1B2C3 were as follows： solution concentration was 0.4 g/mL， the clarifying temperature was 40 ℃ and the addition of chitosan was 0.1%. Conclusion The optimized refining process is stable and feasible. 　　Key words： Bushen Yangxue Granules； icariin； chitosan； refining process； orthogonal test 　　1 仪器与试药 　　Waters Alliance高效液相色谱仪（美国Waters公司），1515型检测泵，2487型检测器，717型自动进样器；BP211D型十万分之一电子天平，BSS210型万分之一电子天平（北京赛多利斯天平有限公司）；Agilent 8453E型紫外分光光度计；DZKW-4型电子恒温水浴锅（北京中兴伟