子宫内膜异位症患者腹腔液中IGFⅠ和PAPPA测定及相关性研究.docVIP

子宫内膜异位症患者腹腔液中IGFⅠ和PAPPA测定及相关性研究 　　[摘要] 目的 检测子宫内膜异位症（EMs）患者和对照组腹腔液中IGF-Ⅰ和PAPP-A的水平变化及相关性，探讨其在子宫内膜异位症发病机制中的作用。方法 采用酶联免疫吸附法（ELISA）检测39例内异症患者（EMs组）及25例非内异症患者（对照组）腹腔液中IGF-Ⅰ和PAPP-A水平。结果 ①EMs组中，IGF-Ⅰ和PAPP-A水平分别为（117.1±36.6）ng/L、（2.85±0.56）ng/mL，均高于对照组的（79.5±18.7）ng/L、（1.93±0.56）ng/mL（P＜0.05）；②Ⅲ～Ⅳ期患者腹腔液中IGF-Ⅰ水平为（126.5±39.1）ng/L高于Ⅰ～Ⅱ期患者的（107.3±32.0）ng/L（P＞0.05），而Ⅲ～Ⅳ期患者腹腔液中PAPP-A水平为（3.15±0.50）ng/mL高于Ⅰ～Ⅱ期患者的（2.55±0.45）ng/mL（P＜0.05），且PAPP-A水平与r-AFS评分呈正相关；③EMs患者腹腔液中PAPP-A和IGF-Ⅰ水平无显著相关性（P＞0.05）。结论 腹腔液中IGF-Ⅰ和PAPP-A的增高可能与内异症的发病有关；PAPP-A与疾病的严重程度有关。 　　[关键词] 子宫内膜异位症；腹腔液；PAPP-A；IGF-Ⅰ 　　[中图分类号] R711.71 [文献标识码] B [文章编号] 1673-9701（2011）23-110-03 　　 　　IGF-Ⅰ and PAPP-A Changes in the Peritoneal Fluid and the Relationship in the Patients of Endometriosis 　　JIN Haihong1 LIU Huining2 ZENG Baojin1 　　1.Department of Obstetrics and Gynecology，Zhejiang Province Taizhou Hospital， Taizhou 317000，China；2. Department of Obstetrics and Gynecology，Hunan Province Changsha Xiangya Hospital，Changsha 410008，China 　　 　　[Abstract] Objective To detect the concentration of IGF-Ⅰand PAPP-A in the peritoneal fluid of women with the endometriosis，so as to investigate the role of PAPP-A and IGF-I in the pathogenesis of endometriosis. Methods The study group included 39 samples of peritoneal fluid from patients with endometriosis，and control group included 25 samples of peritoneal fluid from patients without endometriosis. The peritoneal fluids were collected at the time of laparoscopic operation. Concentration of IGF-Ⅰand PAPP-A were determined by enzyme linked immunosorbent assay（ELISA）. Results ①The peritoneal fluid concentrations of IGF-Ⅰ and PAPP-A in study group were significantly higher than that of control group（117.1±36.6）ng/L vs （79.5±18.7）ng/L， （2.85±0.56）ng/mL vs （1.93±0.56） ng/mL（P＜0.05）；②PAPP-A levels of severe endometriosis were significantly higher than that of mild endometriosis（3.15±0.50）ng/mL vs （2.55±0.45）ng/mL（P＜0.05）. Significant correlation was found between the stages of endometrios