张语迟-高速逆流色谱相的启发和思考.ppt

实例5 二维CCC分离冬凌草 HSCCC条件： 溶剂系统: 一维:正己烷-乙酸乙酯-甲醇-水 1:5:1:5 二维:正己烷-乙酸乙酯-甲醇-水 3:5:3:5 流 速 :2ml min?1 转 速 :800 rpm 样 品 量 :250 mg 粗提物 保 留 值:一维: 40% 二维: 63% 1: oridonin (甲素) 95% 2: ponicidin (乙素) 95% Journal of Chromatography A, 1146 (2007) 125–130 二维逆流色谱分离中药应用 实例6 分离白花前胡中香豆素 HSCCC条件： 溶剂系统: 石油醚-乙酸乙酯-甲醇-水 5:5:6:4 流 速 :2ml min?1 转 速 :900 rpm 样 品 量 :250 mg 粗提物 机 型 :TBE-300 保 留 值 :54.3% 1: 7.0 mg 99.3% 2: 7.4 mg 96.1% 3: 12.5mg 96.5% 4: 42.1mg 99.3% 5: 9.8 mg 99.7% 6: 65.2 mg 98.1% 7: 31.7mg, 92.8% Journal of Chromatography B, 877 (2009) 2571–2578 HSCCC-ESI-MSn分离中药应用 扩展和想象 湖州:活猪走进去 鲜肉运出来 全机械化操作 《中国猪业网》2010-12-27 由于该定点屠宰迁建项目采用自动化同步流水线加工，活猪进去，出来便是新鲜的猪肉，减少了手工的环节和过程，节省了大量人力物力。同时，猪肉更卫生，肉品质量也会有大幅提高。 Application of accelerated solvent extraction coupled with high-performance counter-current chromatography to extraction and online isolation of chemical constituents from Hypericum perforatum L. Yuchi Zhang, Chunming Liu, Min Yu, Zhengkun Zhang, Yanjuan Qi, Jing Wang, Guimei Wu, Sainan Li, Jing Yu, Yang Hu Journal of Chromatography A, 1218 (2011) 2827–2834 Hypericum perforatum L., a typical traditional Chinese medicine, is used all over the world for the treatment of depression. Additionally, it has been reported that the extracts of H. perforatum may have an inhibitory effect, on human bladder cancer cells , and act as an antioxidant and anticonvulsant. The extracts of H. perforatum contain many constituents with documented biological activity such as quinic acids, phloroglucinols and a broad range of flavonoids . Exposure of the extracts of H. perforatum to light may lead to the degradation of phloroglucinols, which are extremely sensitive to oxidation and unstable in solution on exposure to air, therefore phloroglucinols are difficult to separate and isolate by conventional method. In this case, amethod combi