水稻染色质重塑因子CHR729对激素代谢影响.docVIP

水稻染色质重塑因子CHR729对激素代谢影响 　　摘要：CHR729是水稻（Oryza sativa L.）CHD3类染色质重塑因子，广泛参与水稻生长发育进程，并影响全基因组明显表达和组蛋白修饰，测定了多种植物内源激素在chr729突变体中的含量，探讨了CHR729与激素代谢之间的联系。结果表明，在苗期地上部分和成熟期剑叶中，chr729突变体内源生长素（IAA），脱落酸（ABA），茉莉酸（JA）含量明显降低，生长素合成以及ABA合成基因的表达在chr729突变体中均呈下调表达。树脂切片结果显示，chr729突变体中茎秆成熟组织细胞明显变小，居间分生组织细胞大小无明显变化。苗期检测细胞分裂素氧化酶/脱氢酶（CKX）基因表达发现，chr729突变体中CKX1、CKX2、CKX4基因表达量升高，伴随一些位点组蛋白变体H2A.Z富集程度的上升，表明CHR729可能抑制H2A.Z在基因组上的装载，广泛参与水稻激素代谢调控。 　　关键词：水稻（Oryza sativa L.）;染色质重塑因子;激素;干旱 　　中图分类号：Q756 文献标识码：A 文章编号：0439-8114（2015）02-0468-06 　　DOI：10.14088/j.cnki.issn0439-8114.2015.02.055 　　Effects of Rice Chromatin Remodeler CHR729 on Hormone Metabolism 　　LI Jian-jian 　　（College of Life Science and Technology， Huazhong Agricultural University， Wuhan 430070， China） 　　Abstract：CHR729 is a CHD3 chromatin remodeler playing multiple roles in growth and development of rice. Previous studies indicated that CHR729 modulate a subset of gene expression and histone modifications. The contents of multiple endogenous phytohormones were measured. The correlation of CHR729 with hormone dynamics was investigated. A significant decrease in endogenous auxin， abscisic acid and jasmonic acid was observed in chr729 seedlings and mature flag leaves. Genes involved in auxin or abscisic acid biosynthesis were down-regulated in chr729 mutant. The cell length in mature tissue and intercalary meristem in stem was examined. A significant decrease in cell length of mature tissue of chr729 was found. Cell size of intervening meristem had no significant changes. Some cytokinin oxidase/dehydrogenase genes were up-regulated in chr729 mutant， with a relatively high enrichment of histone variant H2A.Z at some loci， indicating the involvement of CHR729 in the deposition/removal of H2A.Z. It is implied that CHR729 was involved in multiple hormone metabolisms of rice. 　　Key words： rice; chromatin remodeler; phytohormone; drought 　　植物激素是植物合成产生的一类小分子化合物。截至目前，多种分子被认定为植物激素，包括生长素或吲哚乙酸（IAA）、细胞分裂素、脱落酸（ABA）、赤霉素、乙烯、油菜素甾醇、茉莉酸、水杨