“鼻塞伤脾”的实验研究-对大鼠消化吸收功能的影响-中医五官科学专业论文.docxVIP

2004 2004 级硕士学位论文 2004 2004 级硕士学位论文 I I II II 摘 要 目的：本文在导师创立并被普遍承认的“鼻塞伤脾”理论指导下，研究探 讨“鼻塞伤脾”对大鼠消化吸收功能的影响，并观察健脾通窍汤的作用。 方法：采用人工堵塞大鼠鼻腔方法，建立实验性鼻塞大鼠模型。造模 14 天后，用健脾通窍汤低、中、高分别三个剂量灌胃治疗，并设立①空白组②模 型组（即鼻塞组）③健脾通窍汤低剂量组（即鼻塞+健脾通窍汤低剂量组）④健 脾通窍汤中剂量组（即鼻塞+健脾通窍汤中剂量组）⑤健脾通窍汤高剂量组（即 鼻塞+健脾通窍汤高剂量组）。治疗 7 天后，观察实验性鼻塞大鼠的一般情况、 体重增长数、脾脏指数、血清胃泌素、胃动素、血清锌、铜离子浓度、红细胞 及血红蛋白计数、光镜下观察脾胃组织等指标的改变。 结果：实验性鼻塞大鼠一般情况较差，体重减轻，脾脏指数降低，血清胃 泌素胃动素含量降低，血清铜离子浓度下降，锌离子浓度降低，血红蛋白和红 细胞数目均有所减少，光镜下观察脾小体数目减少，脾胃粘膜水肿、瘀血。经 过健脾通窍汤低、中、高剂量治疗后，上述病理改变均有不同程度的改善。 结论：“鼻塞伤脾”大鼠消化吸收功能发生改变，适当剂量的健脾通窍汤 可有效地改善相关情况。 关键词：鼻塞伤脾 脾脏指数 胃泌素 胃动素 锌离子 铜离子 红细胞 血 红蛋白 脾胃 健脾通窍汤 Abstract OBJECTIVE: The theory, “spleen should be impaired by nasal obstruction”, is concluded from Pro. Xiong Dajing’s long-clinic experience and admitted extensively. We observe the effect of “spleen should be impaired by nasal obstruction” on absorption and digestion of rats model, meanwhile, the regulation of Jianpitongqiao decoction to this model. METHODS: Establishing rat’s nasal obstruction model by artificial obstructing nasal cavity of rats, 5 groups are involved which are the normal group, the modal group, the group of Jianpitongqiao decoction in low dosage, the group of Jianpitongqiao decoction in middle dosage, the group of Jianpitongqiao decoction in high dosage. After the model was established, Jianpitongqiao decoction is given to the rat by enema starting from 14th to 21st day. After treatment, general condition, weight, spleen index ,GAS, MOT ,trace element Zn2+ Cu2+ number of red cell ,HB and the pathogenic changes of spleen and stomach were examed. RESULTS：The results are as follow: The model rats have poor general conditions, the weight of them grown slowly, spleen index is decreased, GAS and MOT are reduced in plasma, trace element Zn2+ Cu2+ are dropped while number of red cell and HB decreased, the volume of spleen and stomach minification with congestion in spleen sinus. Jianpitongqiao decoction has significant actions of control the symptoms.. CONCLUSION: “Spleen s