“风药”活血作用的实验研究中医基础理论专业论文.docxVIP

成都中医药大学 成都中医药大学 2010 级硕士研究生学位论文 PAGE PAGE 10 ARSBRACT Purpose:1. Minning the theories and clinical application of wind-expelling herbs treating blood stasis syndrome from the related literature in ancient and modern times for theories and practice basis of wind-expelling herbs activating the blood circulation. 2. Studying on the wind-expelling herbs reversing the hepatic fibrosis of lab rats to explore the mechanism of wind-expelling herbs promoting the blood circulation. Methods:1. literature research method: consult the masterpieces in ancient and modern times on wind-expelling herbs invigorating blood circulation to remove blood stasis, and treatments on the diseases of blood stasis with wind-expelling herbs, with the combination of theoretical knowledge of modern clinic practitioners to generalize the characteristics of wind-expelling herbs and minning the theoretical basis of wind-expelling herbs activating the blood circulation. 2. experimental methodology: SD rats were classified into 5 groups: normal contorl, pathological model, HYPERLINK /s?wd=colchicine colchicine group, xia yu xue tang group and wind herbs (fang feng, qiang huo, baizhi) group. Besides the normal group, others were made into HF models by CCl4 subcutaneous injection. Meanwhile, feed each group same amount of normal saline by gavage, 8 weeks later, model checking succeeded. After 12 weeks, collected the blood from femoral vain of the rats before killing them to test the serum concentration of HPC-Ⅲ、ColⅣ-C、FN、HA. Microscopic examine the liver histopathological changes of each group by HE coloration. Under uranyl acetate and lead citrate staining, observe the changes of liver cell organelles and nucleus through transmission electron microscope(TEM). Results：1. literature findings：Study on ancient literatures shows that ancient physicians considered most wind herbs have functions like breaking blood stasis and masses, draining blood vessels and meridians, invigorating blood circul