红景天苷对胶质瘤U87―MG细胞增殖和侵袭能力的影响.docVIP

红景天苷对胶质瘤U87―MG细胞增殖和侵袭能力的影响 　　摘要：目的 观察红景天苷对胶质瘤U87-MG细胞增殖和侵袭能力的影响，探讨其诱导U87-MG细胞凋亡机制。方法 不同浓度红景天苷和喜树碱加入U87-MG细胞中，作用24 h后，MTT法测定U87-MG细胞增殖，流式细胞术检测U87-MG细胞凋亡率，Transwell法检测U87-MG细胞侵袭能力，间接荧光标记法测定细胞内活性氧（ROS）水平，Western blot检测U87-MG细胞Caspase-3、Bcl-2、Bax、E-cadherin、N-cadherin和基质金属蛋白酶-9（MMP-9）的表达。结果 与空白对照组比较，各给药组U87-MG细胞生长抑制作用明显，10、50、100 ?g/mL红景天苷作用U87-MG细胞后，细胞侵袭能力显著下降，10、50、100 ?g/mL红景天苷作用U87-MG细胞48 h后均能诱导U87-MG细胞凋亡；ROS水平与红景天苷浓度呈正相关；10、50、100 ?g/mL红景天苷上调Caspase-3、Bax和E-cadherin的表?_，下调Bcl-2和N-cadherin和MMP-9的表达。结论 红景天苷可诱导U87-MG细胞凋亡、抑制U87-MG细胞的侵袭能力。 　　关键词：红景天苷；胶质瘤U87-MG细胞；细胞增殖；活性氧；Caspase-3；基质金属蛋白酶-9 　　DOI：10.3969/j.issn.1005-5304.2017.06.016 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）06-0064-04 　　Abstract： Objective To investigate the effects of salidroside on proliferation and invasive ability of glioma U87-MG cells； To discuss the its mechanism to induce apoptosis of U87-MG cells. Methods U87-MG cells were cultured in vitro for 24 h under different concentrations of salidroside and camptothecin. The proliferation of U87-MG cells was detected by MTT assay. The apoptosis rate of U87-MG cells was detected by flow cytometry. Transwell assay was used to detect the invasive ability of U87-MG cells. ROS was detected by indirect fluorescent labeling. The expressions of Caspase-3， Bcl-2， Bax， E-cadherin， N-cadherin and matrix metalloproteinase-9 （MMP-9） in U87-MG cells were detected by Western blot. Results Compared with the blank control group， U87-MG cells had significant inhibitory effect on the growth of U87-MG cells in each administration group， and the invasive ability of U87-MG cells was significantly reduced after 10， 50， 100 μg/mL salidroside was intervened， and 10， 50， 100 μg/mL salidroside for 48 h for U87-MG cells could induce apoptosis of the cells； the level of ROS was positively correlated with the concentration of salidroside； 10， 50， 100 μg/mL salidroside up-regulated the expressions of Caspase-3， Bax and E-cadherin， and down-regulated the expressions of Bcl-2， N