芦笋茎枯病菌对甲基托布津的抗药性初步研究探究 .pdfVIP

　J ou rnal of N anj ing A g ricu ltu ral U niversity 芦笋茎枯病菌对甲基托布津的抗药性初步研究 陈新建　叶钟音 (南京农业大学农业部病虫监测与治理重点开放实验室, 南京 210095) 摘　要　经检测江苏芦笋主产区丰县和赣榆芦笋茎枯病菌对甲基托布津已发生严重抗药性。丰县和赣榆田间抗性菌株比 ( ) ( ) ( ) 例为 682% 和 889% , 存在低抗 5 ≤20 、中抗 20 ≤100 和高抗 100 突变体。 EC 50 g mL EC 50 g mL EC 50 g mL 抗药菌株在不含药的培养基中经单孢分离连续培养 8 代, 抗性仍保持稳定。高抗菌株对噻菌灵、苯菌灵、多菌灵表现正交 互抗性, 对乙霉威表现敏感; 低抗、中抗菌株对乙霉威不敏感。抗药菌株的产孢力、菌丝生长速率与敏感菌株相近。 关键词　芦笋茎枯病菌; 甲基托布津; 抗药性 分类号　 436 44 ∶ 6446 S S Prel im inary study on resistance of P hom ap s is . - asp a rag i Sacc to th iophana te m ethyl Chen X in jian and Ye Zhongyin (Key L ab of M on ito ring and M anagem en t of P lan t D iseases and In sects, M in istry of A gricu latu re, N an jing A gric U n iv , N an jing 210095) 　 . , ABSTRACT T he samp les from field pathogen population of P hom ap sis asp arag i Sacc in Fengxian and Ganyu J iangsu ( ) P rovince w ere co llected and their resistance to th iophanate m ethyl TM w as tested in v itro T he results show ed that 889% field iso lates from Ganyu, and 682% iso lates from Fengxian w ere resistant. T h ree levels of resistance to TM w ere ( ) (5 ≤20 ) , ( ) (20 ≤100