探讨酶联免疫吸附试验在包虫病诊断中应用价值.docVIP

探讨酶联免疫吸附试验在包虫病诊断中应用价值 　　[摘要] 目的 通过临床病例资料分析探讨酶联免疫吸附试验在包虫病诊断中的应用价值，为今后的临床工作提供思路。方法以该院2014年1月―2014年12月期间收集的150份人血清样本作为该组研究的检测对象，分别采用酶联免疫吸附试验（ELISA）与滴金免疫渗滤法（DIGFA）对该组150份血清进行包虫病检测，其中包括感染包虫病患者、疑似包虫病感染者以及健康人群血清各50份，对比两种检测方法在包虫病临床诊断中的敏感度与特异性。结果 DIGFA法在包虫病患者血清检测中的特异性抗体的敏感度为96%（48/50）、特异度为100%（50/50）；ELISA法在包虫病患者血清检测中的特异性抗体的敏感度为100%（50/50）、特异度为100%（50/50）；DIGFA与ELISA法检测结果符合率为91.67%，两组在特异性抗体的敏感度（χ2=0.141，P=0.24）与特异度（χ2=0.203，P=0.17）方面，差异无统计学意义（P0.05）。结论 DIGFA法与ELISA法在包虫病临床诊断中的敏感度与特异性均较高，其中ELISA法的敏感度更高，但是对检测时间、操作技能以及设备的要求较高，不适合作为现场流调使用。 　　[关键词] 酶联免疫吸附试验；包虫病；诊断 　　[中图分类号] R5 [文献标识码] A [文章编号] 1674-0742（2015）09（b）-0010-03 　　[Abstract] Objective To investigate the value of enzyme linked immunosorbent assay （ELISA） applied to the diagnosis of hydatid disease via analysis of the clinical data so as to provide thoughts for the clinical work in the future. Methods 150 human serum samples（50 samples of patients with hydatid disease， 50 samples of patients suspected with hydatid disease， and 50 samples of healthy people ） collected from our hospital between January 2014 and December 2014 were selected as the subjects and measured by ELISA and dot immunogold filtration assay（DIGFA）， respectively. And the sensitivity and specificity of the two test methods for the clinical diagnosis of hydatid disease were compared. Results The sensitivity and specificity of DIGFA was 96% （48/50）， and 100% （50/50）， respectively， and that of ELISA was 100%（50/50）， 100% （50/50）， respectively， which showed that the coincidence of test results of the two methods was 91.67%. No statistically significant difference was found between the two groups in sensitivity（χ2=0.141， P=0.24） and specificity（χ2=0.203， P=0.17） （P0.05）. Conclusion Both DIGFA and ELISA have high sensitivity and specificity in the diagnosis of hydatid disease. Compared with DIGFA， ELISA has higher specificity， but it is not suitable for field use because it needs higher requirement in test time， operating skills and equipment. 　　[Key words