组织特异性cxcr4-sirna对前列腺癌骨转移影响的实验研究-experimental study on the effect of tissue - specific cxc r4 - sirna on bone metastasis of prostate cancer.docx

华 华 中 科 技 大 学 博 士 学 位 论 文 I I 目 录 HYPERLINK \l _bookmark0 中文摘要 ································································································· 1 HYPERLINK \l _bookmark1 ABSTRACT 4 HYPERLINK \l _bookmark2 前 言 ···································································································· 8 HYPERLINK \l _bookmark3 第一部分 CXCR4/SDF-1 通路在前列腺癌靶向性转移中作用的初步研究············· 12 HYPERLINK \l _bookmark4 材料和方法 ························································································· 12 HYPERLINK \l _bookmark5 结 果 ································································································ 24 HYPERLINK \l _bookmark6 讨 论 ································································································ 30 HYPERLINK \l _bookmark7 参考文献 ···························································································· 32 HYPERLINK \l _bookmark8 第二部分 携带报告基因 EGFP 的 CXCR4 RNA 干扰质粒的构建及其对前列腺癌细 HYPERLINK \l _bookmark8 胞 CXCR4 基因表达的抑制作用 33 HYPERLINK \l _bookmark9 材料和方法 ························································································· 33 HYPERLINK \l _bookmark10 结 果 ································································································ 45 HYPERLINK \l _bookmark11 讨 论 ································································································ 48 HYPERLINK \l _bookmark12 参考文献 ···························································································· 51 HYPERLINK \l _bookmark13 第三部分 hTERT 启动子调控的 CXCR4-SIRNA 逆转录病毒载体构建及其体外靶向抑 HYPERLINK \l _bookmark13 制 hTERT+前列腺癌细胞生物学效应研究 53 HYPERLINK \l _bookmark14 材料和方法 ························································································· 54 HYPERLINK \l _bookmark15 结 果 ································································································ 70 HYPERLINK \l _bookmark16 讨 论 ············································