【毕业论文】大野荞清除活性氧作用研究.docVIP

李红梅：大野荞清除活性氧作用研究 毕节学院本科毕业论文 第 PAGE 2 页 共 10 页 第 PAGE 1 页 共 10 页 毕业论文 题 目：大野荞清除活性氧作用研究 姓 名： 学 号： 08110902012 教 学 院： 地理与生命科学学院 专业班级： 生物科学2009级 指导教师： （副教授） 完成时间： 2013年05月06日 毕节学院教务处制 毕节学院本科毕业论文 大野荞清除活性氧作用研究 作者姓名： 专业班级：生物科学2009级 摘 要：用NBT的光还原原理、Fenton体系产生的羟自由基与番红花红T作用后使体系吸光度降低、H2O2能和钼酸铵反应形成黄色络合物等原理研究了大野荞各器官（花、叶、茎、根、种子）水提液对羟基自由基（·OH）、超氧阴离子自由基（·O2–）和过氧化氢(H2O2)的清除作用。结果发现：花对·OH的清除作用极显著高于种子，与叶、茎之间无显著差异；根对·O2-的清除作用与花无显著差异，但都极显著高于叶、种子和茎；叶对H2O2的清除作用与花无显著差异，但都极显著高于根、种子和茎。 关键词：大野荞；·OH；·O2-；H2O2；活性氧 Scavenging of Reactive Oxygen Species by the Extraction of F. megaspartanium Candidate: LI Hong-mei Major: Biology science Student No.: 08110902012 Advisor: Zhang yizhong Abstract: This experiment used the method of nitro blue terra-zolium photochemical reduction, the system of fenton can produce the·OH, which can function with Saffron red T ,and then this system reduces the absorbance, H2O2 and ammonium molybdate can become the yellow soil thing and so on, to study the scavenging of the hydroxyl radical (·OH) , superoxide anion radicals (·O2 - ) and hydrogen peroxide ( H2O2 ) were estimated by aqueous extractions from various organs (flower, leaf, stem, root, seed)of F. megaspartanium. The results showed that: the cleaning effect of the flower on ·OH was more significant than the seed, the flower was not significant in difference between the leaf and stem; The root was not significant in difference in the scavenging effect of ·O2 - compared with the flower, but which was more significant than the leaf, seed and stem; The leaf was not significant in difference in the scavenging effect of H2O2 compared with the flower, but which was more significant than the root, seed and stem. Keywords: F. megaspartanium; ·OH; ·O2-; H2O2; Reactive oxygen species 目 录 TOC \o 1-2 \h \z \u HYPERLINK \l _Toc324168937 引言 PAGEREF _Toc324168937 \h 1 HYPERLINK \l _Toc324168938 1. 材料和方法 2 HYPERLINK \l _Toc352523507 1.1 供试材料 2 HYPERLINK \l _Toc