有氧运动和大豆多肽对高脂饮食大鼠肝脏自由基代谢及肝组织形态影响.docVIP

有氧运动和大豆多肽对高脂饮食大鼠肝脏自由基代谢及肝组织形态影响 　　(1.南通大学体育科学学院，江苏 南通 226007；2.扬州大学体育学院，江苏 扬州 22500 9) 　　 　　摘 要： 目的：研究有氧运动和补充大豆多肽对高脂饮食大鼠自由基代谢和肝细胞超微 结构的影响。方法：雄性SD大鼠60只，随机分为C组、HF组、HE组、HS组、HES组5组。检测 大鼠血清AST、ALT、LDH活性以及肝脏SOD和MDA含量，并对肝组织行超微结构观察。结果： 与C组相比,HF组大鼠血清AST、LDH活性显著增高，血清ALT有上升的趋势，但不明显；肝内M DA含量显著上升，肝内SOD活力显著下降。与HF组相比，HE组和HS组以及HES组血清AST、ALT 和LDH活性显著下降；同时，肝组织MDA水平均显著下降,SOD活力显著上升。结论：研究结果 提示,运动和大豆多肽均可以加快高脂饮食大鼠肝组织自由基的清除,并能有效改善高脂饮食 所致的大鼠肝细胞超微结构的损伤；同时，双因素方差分析表明，运动和大豆多肽对降低高 脂饮食大鼠血清AST、LDH活性和肝内MDA浓度以及增强肝内SOD活力有明显的交互作用。 　　关键词：有氧运动；大豆多肽；脂肪肝；自由基；形态结构 　　中图分类号：G804.32文献标识码：A文章编 号：1007-3612(2009)02-0070-05 　　 　　Effects of Aerobic Exercise and Soy Polypeptide on Free RadicalMetabolize and 　　Morphological Changes of High?fat?diet Rats Livers 　　DENG Yu?qiang?1, JIN Qi?guan?2 　　(1.Sports Science School, Nantong University, Nantong 226007, J iangsu China; 　　2.Sports School, Yangzhou University, Yangzhou 225009, Jia ngsu China) 　　Abstract: Objective: To explore the effects of aerobic exercise and supplement o f soy polypeptide (SPI) on free radical metabolize and ultrastructure of high?f a t?diet rats livers. Methods: 60 SD rats are divided randomly into five groups: Group C，Group HF，Group HE，Group HS，and Group HSE. The activity of AST, ALT a nd LDH in their serum and the contents of MDA and SOD in the liver are tested, a nd the ultrastructure of liver tissue is observed. Results: Compared with GroupC，the activity of AST and LDH in serum of Group HF increases significantly, theALT in serum trend upwards but not obvious. The concentration of MDA in liver i ncreases significantly. The SOD activity in the liver decreases significantly. C ompared with HF，the activity of AST, ALT and LDH in serum ofHE, HS and HES de crease significantly. Meanwhile the concentration of MDA decreased dramatically.The SOD activity in the liver increases significantly. Conclusions: The researc h results indicates that both aerobic exercise and SPI can promote to clear freeradicals and improve the ultra?structure d