辽东楤木对过度训练大鼠心肌线粒体游离钙的影响kgzn4xzi.docVIP

辽东楤木对过度训练大鼠心肌线粒体游离钙的影响 2006年第2l卷第5期 ●研究报道ShortCommunication JournalofTUSVo1.21No.52006囝墨圜 辽东木对过度训练大鼠心肌线粒体游离钙的影响 . 刘建华.常波 摘要:目的:通过建立大鼠过度训练模型,探讨辽东楹木对过度训练大鼠心肌线粒体游离钙的影响及其机制.方法:雄性Wistar大鼠,随机分为3组:安 静组,单纯运动组.楹木加运动组,运动模式为6周递增负荷游泳,期间给相应组大鼠服用辽东楹木提取物,最后一次运动后24h取样测定各组大鼠心 肌线粒体MDA,SOD,Ca2十一Mgz十一ATP酶和游离钙等指标.结果:经过6周递增负荷训练,单纯运动组大鼠运动能力下降,心肌线粒体MDA含量显着增高 (Plt;0.01),游离钙,SOD,Ca2+-Mgz十一ATP酶的活性显着下降(P如.01);训练同时服用辽东楹木提取物的大鼠运动能力较运动组有所提高,楹木组心肌线 粒体MDA低于运动组,但无明显差异,SOD,Ca~--M[g~+-ATP酶的活性明显高于运动组(R=o.05),游离钙显着高于运动组(Plt;0.01).结论:经过6周递增负 荷训练,单纯运动组大鼠呈过度训练状态,心肌线粒体自由基生成增多,抗氧化酶活性下降,导致线粒体膜发生脂质过氧化,Ca2*--Mg2+--ATP酶活性下 降,线粒体内游离钙下降,影响线粒体的氧化磷酸化过程;辽东槐木可通过提高抗氧化酶活性,改善过度训练心肌相对缺氧状态,减少自由基的生成而 缓解线粒体的脂质过氧化和游离钙的下降,从整体上表现为加快大鼠疲劳的恢复,提高大鼠的运动能力,对预防过度训练起到一定的作用. 关键词:辽东楹木:过度训练;心肌线粒体;游离钙 中图分类号:G804.5文献标识码:A文章编号:1005—0000(2006)05—0423—03 TheInfluenceofAelataontheFreeCalciumoftheRatsCardiacMuscleMitochondriaduringOvertraining LIUJian—huan.CHANGBo (1.Dept.ofPE,ShandongWeifangCollege,Weifang261061,China;2.DepLofBasicCourses,ShenyangInstituteofPhysicalEducation, Shenyang110032,China) Abstract:Objective:TostudytheinfluenceofA.claraonthefreecalciumoftheratscardiacmusclemitochondriaduringovertrainingbybuildingtherats overtrainingmodelandadministeringratswithA.clarainthisperiod.Method:MaleWistarratsweredividedinto3groupsrandomly:sedentarycontrolgroup ㈧,simplytraininggroup(B),trainingandadministeredwithlowdoseofA.claragroup(c).Thetrainingmodelwasswimmingfor6weeksfollowedincreasing exercisesloadsgradually,whilethecorrespondingratswereadministeredwithA.clara.TheindexessuchasMDA,SOD,Ca~--Mgz._A|rPenzymeandfreecaM- amofthecardiacmusclemitochondriaofratsweremeasuredat24hoursafterlasttraining.Result:After6weekstrainingwithincreasingexercisesloads gradually,comparedwithAgrouprats,theabilitytodoexercisesofBgroupratsdroppedclearly,theindexesofcardiacmusclemitochondriashowedthefol- lowingsignificantlychanges:thecontentofMDAincreasedsignificantly(Plt;O.01),theactivityofSOD,ca2MATPenzymeandtheconcentrationoffree calciumdecreasedsignificantly(Plt;0.